Expressions of Antimicrobial Peptide Gallinacin-1, -2 and -3 mRNAs in the Oviduct of Laying Hens
Expressions of Antimicrobial Peptide Gallinacin-1, -2 and -3 mRNAs in the Oviduct of Laying Hens
复制标题
抗菌肽 Gallinacin-1、-2、-3 mRNA 在蛋鸡输卵管中的表达
DOI：
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Y. Yoshimura
中科院分区：
文献类型：
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作者：
H. Ohashi;K. Subedi;M. Nishibori;N. Isobe;Y. Yoshimura
本研究旨在研究鸡抗菌肽gallinacin（Gal）-1、-2和-3在产蛋鸡输卵管中的mRNA表达谱。采集白色来航蛋鸡输卵管组织。半定量RT-PCR检测Gal-1、Gal-2、Gal-3 mRNA的表达，原位杂交检测Gal-3 mRNA在阴道组织中的表达。RT-PCR检测Gal-1、Gal-2、Gal-3在漏斗部、枕骨大孔、峡部、子宫和阴道中的表达。Gal-1、Gal-2和Gal-3在漏斗部和阴道中的表达均较强，但Gal-2在阴道中的表达较弱。Gal mRNA在阴道上皮基底细胞中均有表达。结果表明，抗菌肽Gal-1、Gal-2和Gal-3在输卵管中均有表达，但在漏斗部和阴道中表达量较大，阴道表面上皮细胞是Gal合成的主要部位。
The aim of this study was to determine the profiles of mRNA expressions of gallinacin (Gal)-1, -2 and -3, which are the antimicrobial peptides, in the oviduct of laying hens. The oviductal tissues of White Leghorn laying hens were collected. Expressions of Gal-1, -2 and -3 mRNA were examined by semiquantitative RT-PCR and localized in the vagina by in situ hybridization. The expressions of Gal-1, -2 and -3 were observed in infundibulum, magnum, isthmus, uterus and vagina by RT-PCR. The expressions of Gal-1, -2 and -3 were greater in the infundibulum and vagina, except for Gal-2 in the vagina. The signals of each Gal mRNA were identified in the basal cells of surface epithelium of vagina. These results suggested that antimicrobial peptide, Gal-1, -2 and -3 were expressed in the oviduct with a greater expression in the infundibulum and vagina, and surface epithelium were the major site for the synthesis of Gals in the vagina.
DOI：
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发表时间：
1998-05
期刊：
The American journal of pathology
影响因子：
--
作者：
A. Quayle;E. Porter;Aimee A. Nussbaum;Yun-Mei Wang;C. Brabec;K. Yip;S. Mok
通讯作者：
A. Quayle;E. Porter;Aimee A. Nussbaum;Yun-Mei Wang;C. Brabec;K. Yip;S. Mok
影响因子：
15.9
作者：
Valore, EV;Park, CH;Ganz, T
通讯作者：
Ganz, T
DOI：
10.1073/pnas.96.2.651
发表时间：
1999-01-19
影响因子：
11.1
作者：
Lillard, JW;Boyaka, PN;McGhee, JR
通讯作者：
McGhee, JR