myb provides an essential function during Drosophila development.
myb provides an essential function during Drosophila development.
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myb 在果蝇发育过程中提供重要功能。
DOI：
10.1073/pnas.93.24.13955
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发表时间：
1996
影响因子：
11.1
通讯作者：
Bishop,JM
中科院分区：
文献类型：
--
作者：
Katzen,AL;Bishop,JM
脊椎动物c-myben编码的转录因子被认为在细胞周期中发挥重要作用。为了进一步了解基因的功能，我们一直在研究果蝇中的一个相关基因。我们发现果蝇mybis在整个发育过程中在所有有丝分裂活跃的组织中大量表达，在一些有丝分裂后组织中表达水平较低，但在多倍体幼虫组织中没有检测到。我们进行了遗传筛查，以分离出包括myb基因在内的染色体区域的隐性致死突变。我们获得了myb的两个温度敏感等位基因，表明该基因提供了一个重要的功能。对突变表型的研究表明，果蝇mybis对胚胎和成虫发育都很重要，mybis在许多组织的发育和卵子发生中起作用。
Vertebrate c-mybencodes a transcription factor thought to play an important role in the cell cycle. To gain further insight intomybfunction, we have been studying a related gene inDrosophila. We found thatDrosophila mybis abundantly expressed throughout development in all mitotically active tissues, at lower levels in some postmitotic tissues, but not at detectable levels in polyploid larval tissues. We performed genetic screens to isolate recessive lethal mutations in the chromosomal region that includes themybgene. We obtained two temperature-sensitive alleles ofmyb, demonstrating that the gene provides an essential function. Examination of the mutant phenotype revealed thatDrosophila mybis important for both embryonic and imaginal development and thatmybserves a role in the development of many tissues and during oogenesis.