The effect and mechanism of urban fine particulate matter (PM2.5) on horizontal transfer of plasmid-mediated antimicrobial resistance genes
The effect and mechanism of urban fine particulate matter (PM2.5) on horizontal transfer of plasmid-mediated antimicrobial resistance genes
复制标题
城市细颗粒物（PM2.5）对质粒介导的抗菌药物耐药基因水平转移的影响及机制
DOI：
10.1016/j.scitotenv.2019.05.115
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发表时间：
2019
影响因子：
9.8
通讯作者：
Chen Jianmin
中科院分区：
文献类型：
--
作者：
Xie Shanshan;Gu April Z;Cen Tianyu;Li Dan;Chen Jianmin
细颗粒物（PM2.5）和抗菌素耐药性是全球公共卫生的两大威胁。目前的空气污染研究严重依赖于PM2.5化学和毒性的评估。然而，PM2.5是否以及如何影响抗菌素耐药基因（ARG）在各种环境中的增殖和转移仍然没有答案。研究了城市PM2. 5对ARGs在机会大肠杆菌（Escherichia coli，E.coli）菌株间水平迁移的影响及其可能机制。结果表明，与对照组相比，西安（XA）、上海（SH）和石家庄（SJZ）城市PM2. 5样品诱导了位置和浓度依赖性的接合转移频率的促进。相关的机制也进行了探讨，包括细胞内活性氧（ROS）的形成和随后的氧化应激，SOS反应，膜通透性的变化，并参与水平转移的基因的mRNA表达的变化的诱导。本研究强调了PM2. 5对促进ARG水平转移的作用，并阐明了城市PM2. 5造成的抗生素耐药性风险的机制。这些发现对于了解抗菌素耐药性在各种环境中的传播具有重要价值，并为重新评估空气质量评估实践提供了有价值的信息。
Fine particulate matter (PM2.5) and antimicrobial resistance are two major threats to public health worldwide. Current air pollution studies rely heavily on the assessment of PM2.5chemistry and toxicity. However, whether and how PM2.5affects the proliferation and transfer of antimicrobial resistance genes (ARGs) in various environments has remained unanswered. This study investigated the effects and potential mechanisms of urban PM2.5on the horizontal transfer of ARGs between opportunisticEscherichia coli(E.coli) strains. The results showed that urban PM2.5samples collected from Xi'an (XA), Shanghai (SH), and Shijiazhuang (SJZ) in China induced location- and concentration-dependent promotion of conjugative transfer frequencies compared to the control group. The relevant mechanisms were also explored, including the formation of intracellular reactive oxygen species (ROS) and the subsequent induction of oxidative stress, SOS response, changes in membrane permeability, and alternations in mRNA expression of genes involved in horizontal transfer. This study highlights the effect of PM2.5on promoting the horizontal transfer of ARGs and elucidates the mechanism of the antimicrobial-resistance risks posed by urban PM2.5. These findings are of great value in understanding the transmission of antimicrobial resistance in various environments and provide valuable information for re-evaluating air quality assessment practices.