Chromosomal studies on sphaerodactyl lizards of genera Gonatodes and Coleodactylus (Squamata, Gekkonidae) using differential staining and fragile sites analyses
Chromosomal studies on sphaerodactyl lizards of genera Gonatodes and Coleodactylus (Squamata, Gekkonidae) using differential staining and fragile sites analyses
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DOI：
10.1159/000076300
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发表时间：
2003-01-01
影响因子：
1.7
通讯作者：
Yonenaga-Yassuda, Y
中科院分区：
文献类型：
--
作者：
dos Santos, RML;Bertolotto, CEV;Yonenaga-Yassuda, Y
本文报道了三种球趾壁虎的染色体组型。本文报道了两种绒螯蟹的核型。hasemani由32条近端着丝粒染色体组成，与已发表的该属其他物种的染色体相似。G. humeralis显示多个Ag-NORs与个体内变异，和阳性C-带位于几对染色体的着丝粒和端粒区域。亚马逊鞘指虫（Coleodactylus amazonicus）是迄今为止研究的第一个非球趾类（Gonatodes sphaerodactyl）动物，具有36条近端着丝粒染色体和1对Ag-NORs。在两对中等大小的染色体上检测到脆性位点。humeralis，其中大多数是在BrdU处理的培养制剂中获得的。这些位点可能是G.类肱骨2n = 32和类亚马孙鳄2n = 36核型。我们的研究结果，特别是在Ag-NORs的位置和脆弱的网站的描述，是相关的，在提高我们的知识，染色体进化的事件，在这个非常可变的和鲜为人知的蜥蜴组。版权所有（C）2003 S. Karger AG，基地
The karyotypes of three species of sphaerodactyl gekkonid lizards are described after conventional and differential staining. Karyotypes of Gonatodes humeralis and G. hasemani are formed by a gradual series of 32 acrocentric chromosomes, similar to those already published for other species of the genus. G. humeralis shows multiple Ag-NORs with intra-individual variability, and positive C-bands located at centromeric and telomeric regions of several chromosome pairs. Coleodactylus amazonicus, the first non-Gonatodes sphaerodactyl studied so far karyologically, exhibits 36 acrocentric/subtelocentric chromosomes and a single pair of Ag-NORs. Fragile sites were detected on two medium-sized chromosome pairs in the karyotype of G. humeralis, most of them obtained in BrdU-treated culture preparations. These sites may represent a putative fission/fusion spot involved in the differentiation of G. humeralis-like 2n = 32 and C amazonicus-like 2n = 36 karyotypes. Our results, especially on the location of Ag-NORs and the description of fragile sites, are relevant in improving our knowledge about the events of chromosome evolution in this extremely variable and poorly known group of lizards. Copyright (C) 2003 S. Karger AG, Base