Plakoglobin, or an 83-kD homologue distinct from beta-catenin, interacts with E-cadherin and N-cadherin.
Plakoglobin, or an 83-kD homologue distinct from beta-catenin, interacts with E-cadherin and N-cadherin.
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DOI：
10.1083/jcb.118.3.671
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发表时间：
1992-08
期刊：
影响因子：
--
通讯作者：
Wheelock MJ
中科院分区：
文献类型：
--
作者：
Knudsen KA;Wheelock MJ
E-和N-钙粘蛋白是参与细胞-细胞粘附的钙依赖性细胞表面糖蛋白家族的成员。在细胞外，跨膜钙粘蛋白自缔合，而在细胞内，它们与肌动蛋白为基础的细胞骨架相互作用。已经注意到几种细胞内蛋白质，统称为连环蛋白，与E-和N-钙粘蛋白共免疫沉淀，并且被认为参与将钙粘蛋白连接到细胞骨架。最近已经鉴定出两种连环蛋白：102-kD的粘着斑蛋白样蛋白（α-连环蛋白）和92-kD的果蝇犰狳/斑珠蛋白样蛋白（β-连环蛋白）。在这里，我们表明，斑珠蛋白，或83 kD斑珠蛋白样蛋白，共免疫沉淀和共定位与E-和N-钙粘蛋白。83-kD蛋白在免疫学上不同于92-kD β-连环蛋白，并且由于其分子量，可能代表称为γ-连环蛋白的钙粘蛋白相关蛋白。因此，斑珠蛋白家族的两个不同成员与N-和E-钙粘蛋白相关联，并且与102-kD α-连环蛋白一起，似乎参与将钙粘蛋白连接到基于肌动蛋白的细胞骨架。
E- and N-cadherin are members of a family of calcium-dependent, cell surface glycoproteins involved in cell-cell adhesion. Extracellularly, the transmembrane cadherins self-associate, while, intracellularly, they interact with the actin-based cytoskeleton. Several intracellular proteins, collectively termed catenins, have been noted to co- immunoprecipitate with E- and N-cadherin and are thought to be involved in linking the cadherins to the cytoskeleton. Two catenins have been identified recently: a 102-kD vinculin-like protein (alpha-catenin) and a 92-kD Drosophila armadillo/plakoglobin-like protein (beta-catenin). Here, we show that plakoglobin, or an 83-kD plakoglobin-like protein, co-immunoprecipitates and colocalizes with both E- and N-cadherin. The 83-kD protein is immunologically distinct from the 92-kD beta-catenin and, because of its molecular mass, likely represents the cadherin- associated protein called gamma-catenin. Thus, two different members of a plakoglobin family associate with N- and E-cadherin and, together with the 102-kD alpha-catenin, appear to participate in linking the cadherins to the actin-based cytoskeleton.