LncRNAs as new biomarkers to differentiate triple negative breast cancer from non-triple negative breast cancer.
LncRNAs as new biomarkers to differentiate triple negative breast cancer from non-triple negative breast cancer.
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LncRNA作为区分三阴性乳腺癌和非三阴性乳腺癌的新生物标志物
DOI：
10.18632/oncotarget.7509
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发表时间：
2016-03-15
期刊：
影响因子：
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通讯作者：
Fu Z
中科院分区：
文献类型：
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作者：
Lv M;Xu P;Wu Y;Huang L;Li W;Lv S;Wu X;Zeng X;Shen R;Jia X;Yin Y;Gu Y;Yuan H;Xie H;Fu Z
三阴性乳腺癌（TNBC）是一种具有高度异质性的侵袭性乳腺癌。迄今为止，对于TNBC患者没有有效的治疗方法，并且预后差。寻找新的TNBC诊断标志物或有效的治疗靶点是当务之急。作为后基因组时代的焦点领域，已发现长非编码RNA（lncRNA）在许多癌症（包括TNBC）中起关键作用。然而，关于TNBC和非TNBC之间差异表达的lncRNA的信息很少。我们分别检测了TNBC和非TNBC组织中lncRNA的表达水平。然后我们分析了TNBC与non-TNBC相关的lncRNA表达特征，通过基因本体论和通路分析发现，表达异常的lncRNA参与了重要的生物学过程。最后，我们验证了这些lncRNA在乳腺癌组织和细胞中的表达水平，然后通过受试者工作特征曲线分析证实了4种lncRNA（RP 11 -434D9.1，LINC 00052，BC 016831和IGKV）与TNBC发生相关。本研究为全面了解TNBC的发生发展机制提供了有益的信息，并为临床治疗提供了潜在的诊断生物标志物或治疗靶点。
Triple negative breast cancer (TNBC) is an aggressive type of breast cancer with high heterogeneity. To date, there is no efficient therapy for TNBC patients and the prognosis is poor. It is urgent to find new biomarkers for the diagnosis of TNBC or efficient therapy targets. As an area of focus in the post-genome period, long non-coding RNAs (lncRNAs) have been found to play critical roles in many cancers, including TNBC. However, there is little information on differentially expressed lncRNAs between TNBC and non-TNBC. We detected the expression levels of lncRNAs in TNBC and non-TNBC tissues separately. Then we analyzed the lncRNA expression signature of TNBC relative to non-TNBC, and found dysregulated lncRNAs participated in important biological processes though Gene Ontology and Pathway analysis. Finally, we validated these lncRNA expression levels in breast cancer tissues and cells, and then confirmed that 4 lncRNAs (RP11-434D9.1, LINC00052, BC016831, and IGKV) were correlated with TNBC occurrence through receiver operating characteristic curve analysis. This study offers helpful information to understand the initiation and development mechanisms of TNBC comprehensively and suggests potential biomarkers for diagnosis or therapy targets for clinical treatment.