Development of a novel label-free amperometric immunosensor for the detection of okadaic acid
Development of a novel label-free amperometric immunosensor for the detection of okadaic acid
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DOI：
10.1016/j.aca.2012.02.035
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发表时间：
2012-04-29
影响因子：
6.2
通讯作者：
Marty, Jean-Louis
中科院分区：
文献类型：
--
作者：
Hayat, Akhtar;Barthelmebs, Lise;Marty, Jean-Louis
冈田酸 (OA) 是一种亲脂性藻毒素，主要由产毒甲藻产生。显然，需要开发能够改进传统方法的高性能 OA 分析方法。在这项工作中，开发了一种新的无标记检测 OA 的实验方法。使用 Protein G 磁珠（Protein-G-MBs）修饰金电极固定抗 OA 单克隆抗体（anti-OA-MAb）。进行初步比色测试以验证蛋白-G-MB 和抗 OA-MAb 反应。在铁/亚铁氰化物溶液中通过差分脉冲伏安法进行电化学检测。获得的检测限（0.5 μg L-1）验证了所开发的电化学免疫传感器作为常规使用的有前途的工具。还用实际样品评估了基质效应和回收率，显示出良好的回收率。 (C) 2012 Elsevier B.V. 保留所有权利。
Okadaic acid (OA), a lipophilic phycotoxin is mainly produced by toxigenic dinoflagellates. The need to develop high performing methods for OA analysis able to improve the traditional ones is evident. In this work, a novel experimental methodology for label-free detection of OA was developed. Protein G magnetic beads (protein-G-MBs) modified gold electrode was used to immobilize anti-OA monoclonal antibody (anti-OA-MAb). Preliminary, colorimetric tests were performed in order to validate protein-G-MBs and anti-OA-MAb reaction. Electrochemical detection was carried out by differential pulse voltammetry in ferri/ferrocyanide solution. The limit of detection value obtained (0.5 mu g L-1) validated the developed electrochemical immunosensor as a promising tool for routine use. The matrix effect and the recovery rate were also assessed with real samples, showing a good percentage of recovery. (C) 2012 Elsevier B.V. All rights reserved.