Effects of slurry from sulfadiazine- (SDZ) and difloxacin- (DIF) medicated pigs on the structural diversity of microorganisms in bulk and rhizosphere soil
Effects of slurry from sulfadiazine- (SDZ) and difloxacin- (DIF) medicated pigs on the structural diversity of microorganisms in bulk and rhizosphere soil
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DOI：
10.1016/j.soilbio.2013.03.007
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发表时间：
2013-07-01
影响因子：
9.7
通讯作者：
Thiele-Bruhn, Soeren
中科院分区：
文献类型：
--
作者：
Reichel, Ruediger;Rosendahl, Ingrid;Thiele-Bruhn, Soeren
传统农业仍然消耗大量抗生素，例如磺胺嘧啶（SDZ）或二氟沙星（DIF），以保护牲畜免受传染病的侵害。因此，将药用动物的泥浆施用于可耕地。越来越多的研究表明，抗生素与猪粪混合施用能够改变无根土壤微生物群落结构。根际土壤中的影响，以及药物衍生的直接和间接影响的改变泥浆组成的研究很少。我们评估了在63天的围隔实验中微生物对SDZ和DIF给药猪泥浆的响应，考虑到典型的农业猪泥浆修正案和开发玉米根系的自然复杂性。泥浆来源的粪便细菌仍然存在于围隔土壤14天后，修正案。DIF和SDZ的药物治疗进一步改变了猪泥浆的分子化学模式，混淆了精确的抗生素作用。在生态相关条件下研究抗菌作用时必须考虑这一点。对围隔生态系统土壤中微生物群落的影响与以前直接加标土壤的研究结果广泛匹配。在中生态系统根际土壤中也发现了影响，但并不比散装土壤更明显。在应用人工SDZ加标对照浆液后，也在实验室条件下验证了这一点。(C)2013爱思唯尔有限公司保留所有权利。
Conventional farming still consumes considerable amounts of antibiotics such as sulfadiazine (SDZ) or difloxacin (DIF) to protect livestock from infectious diseases. Consequently, slurries from medicated animals are applied to arable soils. Antibiotics, co-applied with pig slurry, are increasingly reported to change soil microbial community structures in un-rooted bulk soil. The effects in rhizosphere soil, as well as the medication-derived direct and indirect effects of an altered slurry composition are poorly investigated. We evaluated the response of microorganisms to slurry of SDZ- and DIF-medicated pigs in a 63-d mesocosm experiment, considering the natural complexity of a typical agricultural pig slurry amendment and developing Zea mays L root systems. Slurry-derived fecal bacteria were still present in mesosocosm soil 14 days after amendment. Medication with DIF and SDZ further altered the molecular-chemical pattern of the pig slurry, confounding the precise antibiotic effect. This has to be considered when investigating antimicrobial effects under ecological relevant conditions. Effects on the microbial community in mesocosm bulk soil widely matched results from previous studies on directly spiked soil. Effects were also found in the mesocosm rhizosphere soil, but not more pronounced than in bulk soil. This was also verified under laboratory conditions after application of artificially SDZ-spiked control slurry. (C) 2013 Elsevier Ltd. All rights reserved.