MicroRNAs Establish Uniform Traits during the Architecture of Vertebrate Embryos.
MicroRNAs Establish Uniform Traits during the Architecture of Vertebrate Embryos.
复制标题
DOI：
10.1016/j.devcel.2017.02.021
复制
发表时间：
2017-03-27
影响因子：
11.8
通讯作者：
Nicoli S
中科院分区：
文献类型：
--
作者：
Kasper DM;Moro A;Ristori E;Narayanan A;Hill-Teran G;Fleming E;Moreno-Mateos M;Vejnar CE;Zhang J;Lee D;Gu M;Gerstein M;Giraldez A;Nicoli S
Proper functioning of an organism requires cells and tissues to behave in uniform, well-organized ways. How this optimum of phenotypes is achieved during the development of vertebrates is unclear. Here, we carried out a multifaceted and single-cell resolution screen of zebrafish embryonic blood vessels upon mutagenesis of single and multi-gene miRNA families. We found that embryos lacking particular miRNA-dependent signaling pathways develop a vascular trait similar to wild type, but with a profound increase in phenotypic heterogeneity. Aberrant trait variance in miRNA mutant embryos uniquely sensitizes their vascular system to environmental perturbations. We discovered a previously unrecognized role for specific vertebrate miRNAs to protect tissue development against phenotypic variability. This discovery marks an important advance in our comprehension of how miRNAs function in the development of higher organisms. Phenotypic diversity must be controlled to ensure balance between trait functionality and trait adaptability to changing environments. Kasper et al. establish that specific miRNAs limit phenotypic variation of the vascular system in a vertebrate embryo. Altered phenotypic variability resulting from miRNA loss sensitizes blood vessels to diverse environmental stresses.
