Metabolic behavior of acetyl glyceryl ether phosphorylcholine on interaction with rabbit platelets.
Metabolic behavior of acetyl glyceryl ether phosphorylcholine on interaction with rabbit platelets.
复制标题
乙酰甘油醚磷酰胆碱与兔血小板相互作用的代谢行为。
DOI：
10.1016/0003-9861(83)90236-9
复制
发表时间：
1983
影响因子：
3.9
通讯作者：
Hanahan,DJ
中科院分区：
文献类型：
--
作者：
Pieroni,G;Hanahan,DJ
研究了1-O-[3 H]烷基-2-乙酰基-sn-甘油基-3-磷酸胆碱（[3 H]-AGEPC）与兔血小板相互作用后的代谢转归。[3 H]AGEPC转化为一种被鉴定为长链脂肪酰基类似物的产物。该反应不受细胞外钙的影响。在30至60秒的滞后时间之后，转化的动力学是线性的。发现反应速率是血小板和AGEPC浓度的函数。当在37 ℃下与每毫升1.25 × 109个血小板的悬浮液一起孵育时，[3 H]AGEPC（10− 9 m）的85 ± 5%在1小时内被加工成长链脂肪酰基类似物。在2 mmEDTA存在下，每个血小板每分钟最多有1200至3600个[3 H]AGEPC分子转化为长链脂肪酰基衍生物。在类似条件下，1-O-[3 H]烷基-2-（溶血）-sn-甘油基-3-磷酸胆碱（[3 H]lysoGEPC）也以慢得多的速率和较低的程度转化为相当的长链脂肪酰基衍生物。在含有[3 H]AGEPC的孵育混合物中未观察到lysoGEPC的显著增加。AGEPC与血小板相互作用后可能直接转酰化进行了讨论，以及可能参与直接触发血小板对AGEPC刺激的反应。
The metabolic fate of 1-O-[3H]alkyl-2-acetyl-sn-glycero-3-phosphorylcholine ([3H]-AGEPC) upon interaction with rabbit platelets was investigated. [3H]AGEPC was converted to a product identified as the long-chain fatty acyl analog. The reaction was unaffected by extracellular calcium. After a lag time of 30 to 60 s the kinetics of the conversion was linear. The rate of the reaction was found to be a function of platelet and AGEPC concentrations. Of the [3H]AGEPC (10−9m) 85 ± 5% was processed into the-long chain fatty acyl analog within 1 h when incubated at 37 †C with a 1.25 × 109platelets per milliliter suspension. A maximal number of 1200 to 3600 [3H]AGEPC molecules were converted to the long-chain fatty acyl derivative per minute per platelet in the presence of 2 mmEDTA. Under similar conditions the 1-O-[3H]alkyl-2-(lyso)-sn-glycero-3-phosphorylcholine ([3H]lysoGEPC) also was transformed to a comparable long-chain fatty acyl derivative at a much slower rate and to a lower extent. No significant increase in lysoGEPC was noted in incubation mixtures containing [3H]AGEPC. The possible direct transacylation of AGEPC upon interaction with platelets is discussed as well as the possible involvement of this reaction in directly triggering the platelet response to AGEPC stimuli.