Taurine Transporter dEAAT2 is Required for Auditory Transduction in Drosophila
Taurine Transporter dEAAT2 is Required for Auditory Transduction in Drosophila
复制标题
果蝇听觉转导需要牛磺酸转运蛋白 dEAAT2。
DOI：
10.1007/s12264-018-0255-1
复制
发表时间：
2018
影响因子：
5.6
通讯作者：
Yan Zhiqiang
中科院分区：
文献类型：
--
作者：
Sun Ying;Jia Yanyan;Guo Yifeng;Chen Fangyi;Yan Zhiqiang
果蝇adeaat2是兴奋性氨基酸转运蛋白（EAAT）家族的一员，已被描述为介导牛磺酸的高亲和力转运，牛磺酸是昆虫和哺乳动物中丰富的游离氨基酸。然而，牛磺酸及其转运体在听力中的作用尚不清楚。在这里，我们报告了dEAAT2是幼虫对声音刺激的惊吓反应所必需的。结果发现，在声音转导发生的声带神经元远端区域，dEAAT2富集。Ca2+成像和电生理结果显示，被破坏的dEAAT2表达显著降低了chordoonal神经元对声音的反应。更重要的是，在弦状神经元中表达dEAAT2挽救了这些突变表型。综上所述，这些发现表明果蝇adeaat2在脊索神经元的声音转导中起着关键作用。
DrosophiladEAAT2, a member of the excitatory amino-acid transporter (EAAT) family, has been described as mediating the high-affinity transport of taurine, which is a free amino-acid abundant in both insects and mammals. However, the role of taurine and its transporter in hearing is not clear. Here, we report that dEAAT2 is required for the larval startle response to sound stimuli. dEAAT2 was found to be enriched in the distal region of chordotonal neurons where sound transduction occurs. The Ca2+imaging and electrophysiological results showed that disrupted dEAAT2 expression significantly reduced the response of chordotonal neurons to sound. More importantly, expressing dEAAT2 in the chordotonal neurons rescued these mutant phenotypes. Taken together, these findings indicate a critical role forDrosophiladEAAT2 in sound transduction by chordotonal neurons.