WST-8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells
WST-8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells
复制标题
DOI：
10.1007/978-1-61779-108-6_4
复制
发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Stoddart, Martin J.
中科院分区：
文献类型：
--
作者：
Stoddart, Martin J.
WST-8 是新一代甲臜染料之一，它将转化后的产物以可溶形式释放到介质中。这允许非破坏性地确定活力，使细胞能够接受进一步的研究。在研究细胞表型并且基质合成等数据与细胞数量相关的情况下，这是一个主要优势。本章介绍如何使用 WST-8 使碱性磷酸酶活性正常化。
WST-8 is one of the newer generation formazan-based dyes, which release the converted product into the medium in a soluble form. This allows for a non-destructive determination of viability enabling the cells to be subject to further investigations. This is a major advantage in cases where cell phenotype is being investigated and data such as matrix synthesis is correlated to cell number. This chapter describes the use of WST-8 to normalise alkaline phosphatase activity.