Growth of IM-9 human lymphoblasts in serum-free medium: stimulation by glucocorticoids.
Growth of IM-9 human lymphoblasts in serum-free medium: stimulation by glucocorticoids.
复制标题
IM-9 人淋巴母细胞在无血清培养基中的生长：糖皮质激素的刺激。
DOI：
10.1111/j.1365-2184.1988.tb00777.x
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Straus,DS
中科院分区：
文献类型：
--
作者：
Straus,DS
The IM‐9 human B‐lymphoblast cell line grows well in a completely defined serum‐free medium containing insulin, transferrin, low density lipoprotein and oleic acid in complex with fatty acid‐free bovine serum albumin. Growth of the IM‐9 cells is stimulated by addition of physiological concentrations of hydrocortisone to this medium. the order of growth stimulatory potency of several steroids is dexamethasone > hydrocortisone > aldosterone, whereas testosterone does not stimulate growth of the IM‐9 cells. This order of potency suggests that the effect is mediated by binding to glucocorticoid receptors. Growth of the IM‐9 cells is also stimulated by the neuropeptide substance P. the defined serum‐free medium described in this report will be useful for further studies of the biological responses of the IM‐9 cells to other hormones in the absence of interference from hormones and growth factors present in serum.
