The role of peroxidase in the bactericidal activity of human blood eosinophils.
The role of peroxidase in the bactericidal activity of human blood eosinophils.
复制标题
过氧化物酶在人血嗜酸性粒细胞杀菌活性中的作用。
作者：
J. Bujak;R. Root
利用含有90%以上嗜酸性粒细胞和叠氮化钠的细胞制剂抑制溶酶体酶过氧化物酶，研究了嗜酸性粒细胞的杀菌活性。嗜酸性粒细胞不能像中性粒细胞那样杀死细菌，主要是因为其吞噬能力降低。与中性粒细胞相比，嗜酸性粒细胞具有较高的静息碘化活性，但在吞噬作用后不能增加这种活性。一毫莫叠氮化钠可以抑制嗜酸性粒细胞和中性粒细胞过氧化物酶（通过对三氯乙酸可沉淀蛋白的碘化测定），损害中性粒细胞的葡萄球菌活性，但主要通过增加吞噬摄取来增强嗜酸性粒细胞对金黄色葡萄球菌的杀伤。利用溶葡萄球菌蛋白的杀菌研究表明，中性粒细胞过氧化物酶仅在吞噬后早期发挥其杀菌活性。嗜酸性粒细胞过氧化物酶在遗传和生化上不同于中性粒细胞过氧化物酶，似乎在完整的嗜酸性粒细胞的杀菌活性中不起作用。
Utilizing cell preparations that contained greater than 90% eosinophils and sodium azide to inhibit the lysosomal enzyme peroxidase, studies of eosinophil bactericidal activity were undertaken. Eosinophils did not kill bacteria as well as neutrophils, principally because of a reduced phagocytic capacity. In contrast to neutrophils, eosinophils had high resting iodination activity but failed to increase this activity following phagocytosis. One millimolar sodium azide, which inhibits both eosinophil and neutrophil peroxidase as measured by the iodination of trichloroacetic acid precipitable protein, impaired neutrophil staphylocidal activity, but enhanced eosinophil killing of Staphylococcus aureus primarily by increasing phagocytic uptake. Bactericidal studies utilizing lysostaphin suggest that neutrophil peroxidase exerts its contribution to bactericidal activity only during the early postphagocytic period. Eosinophil peroxidase is genetically and biochemically distinct from neutrophil peroxidase, and appears to play no role in the bactericidal activity of intact eosinophils.