Development of a CAPS marker for the Pvr4 locus:: A tool for pyramiding potyvirus resistance genes in pepper
Development of a CAPS marker for the Pvr4 locus:: A tool for pyramiding potyvirus resistance genes in pepper
复制标题
DOI：
10.1139/gen-42-6-1111
复制
发表时间：
1999-12-01
期刊：
影响因子：
3.1
通讯作者：
Palloix, A
中科院分区：
文献类型：
--
作者：
Caranta, C;Thabuis, A;Palloix, A
辣椒中的Pvr 4抗性基因赋予对马铃薯Y病毒（PVY）和辣椒斑驳病毒（PepMoV）的三种致病型的完全抗性。为了利用该基因进行分子标记辅助选择（MAS），并使同一品种中的多个马铃薯Y病毒属抗病基因能够同时扩增，本研究采用混合分离分析方法获得了紧密连锁的AFLP标记。8个连锁AFLP标记定位在该位点周围2.1 +/- 0.8至13.8 +/- 2.9 cM的区间内。使用来自两个等位基因序列的比对的数据，将最接近的共显性AFLP标记转换成共显性CAPS（切割扩增多态性序列）标记。我们进一步表征了CAPS标记在不同辣椒育种系中用于MAS程序的相关性。
The Pvr4 resistance gene in pepper confers a complete resistance to the three pathotypes of potato virus Y (PVY) and to pepper mottle virus (PepMoV). In order to use this gene in a marker-assisted selection (MAS) program and to permit the pyramiding of several potyvirus resistance genes in the same cultivar, tightly linked amplified fragment length polymorphism (AFLP) markers were obtained by the bulked segregant analysis method. Eight linked AFLP markers were mapped in an interval from 2.1 +/- 0.8 to 13.8 +/- 2.9 cM around this locus. The closest codominant AFLP marker was converted into a codominant CAPS (cleaved amplified polymorphic sequence) marker using data from the alignment of the two allele sequences. We have further characterized the relevance of the CAPS marker for MAS programs in different pepper breeding lines.