MHC class I-mediated exogenous antigen presentation by exosomes secreted from immature and mature bone marrow derived dendritic cells
MHC class I-mediated exogenous antigen presentation by exosomes secreted from immature and mature bone marrow derived dendritic cells
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DOI：
10.1016/s0165-2478(03)00128-7
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发表时间：
2003-10-31
影响因子：
4.4
通讯作者：
Minami, M
中科院分区：
文献类型：
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作者：
Utsugi-Kobukai, S;Fujimaki, H;Minami, M
外泌体是具有抗原呈递能力的50-90 nm囊泡，其携带主要组织相容性复合物（MHC）I类、II类、丰富的共刺激分子和一些tetraspan蛋白。树突状细胞（dendritic cells，DC）是一种能够以MHC I类分子介导的抗原特异性方式（交叉呈递）呈递外源性抗原的专职抗原呈递细胞，但未成熟或成熟DC的外泌体的交叉呈递能力尚不清楚。在这里，我们表明，从卵清蛋白（OVA）蛋白脉冲骨髓来源的树突状细胞（BM-DC）释放的外泌体弱地将肽决定簇呈递给OVA特异性MHBC I类限制性CD 8（+）T细胞杂交瘤。由OVA（257-264）肽或OVA蛋白脉冲的成熟BM-DC分泌的外泌体比来自未成熟BM-DC的外泌体更有效地激活OVA特异性MHC I类限制性T细胞杂交瘤。还使用与抗原加工（TAP）缺陷型小鼠衍生的BM-DC相关的转运蛋白来检查功能性TAP活性是否是外泌体交叉呈递所需的。从来源于TAP（-/-）小鼠的OVA（257-264）肽脉冲的BM-DC获得的外来体显示出对OVA特异性MHC I类限制性T细胞杂交瘤的显著抗原呈递能力。总之，我们的数据表明BM-DCs分泌具有弱交叉呈递能力的外泌体。(C)2003 Elsevier B. V.保留所有权利。
Exosomes are 50-90 nm vesicles with antigen presenting ability carrying major histocompatibility complex (MHC) class I, class II, abundant co-stimulatory molecules and some tetraspan proteins. Although dendritic cells (DCs) are one of the professional antigen presenting cells capable of presenting exogenous antigens in MHC class I-mediated antigen specific manner (cross-presentation), the cross-presentation ability by exosomes from immature or mature DCs are unknown. Here we show that exosomes released from ovalbumin (OVA) protein-pulsed bone marrow derived dendritic cells (BM-DCs) weakly present the peptide determinants to OVA specific MHBC class I-restricted CD8(+) T cell hybridomas. The exosomes secreted by OVA(257-264) peptide- or OVA protein-pulsed mature BM-DCs activated OVA specific MHC class I-restricted T cell hybridomas more efficiently than those from immature BM-DCs. Transporters associated with antigen processing (TAP) deficient mice-derived BM-DCs were also used to examine whether functional TAP activity was required for cross-presentation by exosomes. The exosomes obtained from OVA(257-264) peptide-pulsed BM-DCs derived from TAP(-/-) mice showed a significant antigen presenting ability to OVA specific MHC class I-restricted T cell hybridomas. Altogether, our data indicate that BM-DCs secrete exosomes with weak cross-presentation ability. (C) 2003 Elsevier B.V. All rights reserved.