Identification of two novel proteins that interact with germ-cell-specific RNA-binding proteins DAZ and DAZL1
Identification of two novel proteins that interact with germ-cell-specific RNA-binding proteins DAZ and DAZL1
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DOI：
10.1006/geno.2000.6169
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发表时间：
2000-05-01
期刊：
影响因子：
4.4
通讯作者：
Yen, PH
中科院分区：
文献类型：
--
作者：
Tsui, SL;Dai, T;Yen, PH
Y染色体上的人类DAZ(在无精子症中缺失)基因家族和一个常染色体DAZ样基因DAZL1编码RNA结合蛋白，这些蛋白仅在生殖细胞中表达。它们在精子发生中的作用得到了它们与果蝇雄性不育基因Boule和Dazl1基因敲除小鼠的不育的同源性的支持。虽然所有哺乳动物的常染色体上都含有DAZL1同源基因，但DAZ同源基因只存在于类人猿和东半球猴子的Y染色体上。DAZ和DAZL1蛋白在DAZ重复序列和C末端序列的拷贝数上有所不同。我们研究了DAZ和DAZL1与其他蛋白质的相互作用，以此来研究这两个蛋白质之间的功能相似性。在酵母双杂交系统中，以DAZ为诱饵，分离了两个DAZAP(DAZ相关蛋白)基因。DAZAP1编码一种新的RNA结合蛋白，在睾丸中表达最丰富，DAZAP2编码一种普遍表达的蛋白，没有可识别的功能基序。DAZAP1和DAZAP2通过DAZ重复序列与DAZ和DAZL1类似地结合。DAZAP基因分别定位在染色体19p13.3和2q33-q34区域，尚未发现影响精子发生的遗传性疾病。(C)2000年学术出版社。
The human DAZ (deleted in azoospermia) gene family on the Y chromosome and an autosomal DAZ-like gene, DAZL1, encode RNA-binding proteins that are expressed exclusively in germ cells. Their role in spermatogenesis is supported by their homology with a Drosophila male infertility gene boule and sterility of Dazl1 knock-out mice. While all mammals contain a DAZL1 homologue on their autosomes, DAZ homologues are present only on the Y chromosomes of great apes and Old World monkeys. The DAZ and DAZL1 proteins differ in the copy numbers of a DAZ repeat and the C-terminal sequences. We studied the interaction of DAZ and DAZL1 with other proteins as an approach to investigate functional similarity between these two proteins. Using DAZ as bait in a yeast two-hybrid system, we isolated two DAZAP (DAZ-associated protein) genes. DAZAP1 encodes a novel RNA-binding protein that is expressed most abundantly in the testis, and DAZAP2 encodes a ubiquitously expressed protein with no recognizable functional motif. DAZAP1 and DAZAP2 bind similarly to both DAZ and DAZL1 through the DAZ repeats. The DAZAP genes were mapped to chromosomal regions 19p13.3 and 2q33-q34, respectively, where no genetic diseases affecting spermatogenesis are known to map. (C) 2000 Academic Press.