Ring trial for the simultaneous analysis of sweeteners and preservatives in soft drinks
Ring trial for the simultaneous analysis of sweeteners and preservatives in soft drinks
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DOI：
10.3920/qas2012.0124
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发表时间：
2013-02
影响因子：
4
通讯作者：
H. Ozer;V. Psimouli;N. Ozcan;B. Ozer;I. Papadaki;V. Oreopoulou
中科院分区：
文献类型：
--
作者：
H. Ozer;V. Psimouli;N. Ozcan;B. Ozer;I. Papadaki;V. Oreopoulou
本文介绍了同时测定软饮料中三种人工甜味剂（乙酰磺胺k、阿斯巴甜和糖精）和两种防腐剂（山梨酸和苯甲酸）的环试验。根据EN 12856:1999方法，采用高效液相色谱法进行分析。11个实验室参与了环形试验。结果表明，该方法可同时测定所选甜味剂和防腐剂的含量。阿斯巴甜的重复性相对标准偏差为2.26%，乙酰磺胺- k的重复性相对标准偏差为5.05%，糖精钠的重复性相对标准偏差为2.38%，苯甲酸的重复性相对标准偏差为3.72%，山梨酸的重复性相对标准偏差为7.93%，目标物的重复性相对标准偏差分别为7.5、6.3、4.5、11.0和7.2%。根据对橙汁饮料、橙味饮料和橙汁的实验室间测试，这三种甜味剂的精度数据与EN 12856:1999报告的各自值相当。
In the present study a ring trial on the simultaneous determination of three artificial sweeteners (acesulfame-K, aspartame and saccharin) and two preservatives (sorbic and benzoic acid) in a soft drink is presented. High performance liquid chromatography was used for the analysis, according to the EN 12856:1999 method. Eleven laboratories participated in the ring trial. The method proved to be efficient for the simultaneous determination of the selected sweeteners and preservatives in one run analysis. The repeatability relative standard deviation was 2.26% for aspartame, 5.05% for acesulfame-K, 2.38% for sodium saccharin, 3.72% for benzoic acid, 7.93% for sorbic acid and the reproducibility relative standard deviation of the target analytes was 7.5, 6.3, 4.5, 11.0 and 7.2% respectively. The precision data for the three sweeteners were comparable to the respective values reported by the EN 12856:1999 according to inter-laboratory tests in orange juice beverage, orange flavoured beverage and orange juice.