Occludin expression in goldfish held in ion-poor water
Occludin expression in goldfish held in ion-poor water
复制标题
DOI：
10.1007/s00360-008-0297-1
复制
发表时间：
2009-02-01
影响因子：
2
通讯作者：
Kelly, Scott P.
中科院分区：
文献类型：
--
作者：
Chasiotis, Helen;Effendi, Jennifer C.;Kelly, Scott P.
以紧密连接蛋白occludin为重点，研究了金鱼在突然暴露（0-120 h）以及长期驯化（14 d和28 d）后对贫离子水（IPW）的反应。突然和长期暴露于IPW均降低血清渗透压、[Na+]和[Cl-]，并升高血清葡萄糖。IPW突然暴露后，鳃组织Na+-K+-ATPase活性迅速持续下降，occludin表达短暂增加，6 h后恢复到对照水平。IPW后14和28天，鳃occludin表达显着升高，而Na+-K+-ATP酶活性仅在第14天显着不同（升高）。肾脏组织表现出的Na+-K+-ATP酶活性和occludin表达的28天后，升高，然而，在肠道中，occludin表达下降，在第14天，但没有不同的FW鱼在第28天。这些研究表明，金鱼可以忍受突然以及持续暴露于离子贫乏的环境。数据还表明，occludin可能发挥适应性的作用，在鱼类适应离子缺乏的条件下，通过促进上皮细胞的离子调节组织的屏障性能的调制。
With an emphasis on the tight junction protein occludin, the response of goldfish following abrupt exposure (0-120 h) as well as long-term acclimation (14 and 28 days) to ion-poor water (IPW) was examined. Both abrupt and long-term exposure to IPW lowered serum osmolality, [Na+] and [Cl-], and elevated serum glucose. After abrupt exposure to IPW, gill tissue exhibited a prompt and sustained decrease in Na+-K+-ATPase activity, and a transient increase in occludin expression that returned to control levels by 6 h. Following 14 and 28 days in IPW, gill occludin expression was markedly elevated, while Na+-K+-ATPase activity was only significantly different (elevated) at day 14. Kidney tissue exhibited an elevation in both Na+-K+-ATPase activity and occludin expression after 28 days; however, in the intestine, occludin expression declined at day 14 but did not differ from FW fish at day 28. These studies demonstrate that goldfish can tolerate abrupt as well as sustained exposure to ion-poor surroundings. Data also suggests that occludin may play an adaptive role in fishes acclimated to ion-poor conditions by contributing to the modulation of epithelial barrier properties in ionoregulatory tissues.