Contrasts in the actions of protein antibiotics on deoxyribonucleic acid structure and function.
Contrasts in the actions of protein antibiotics on deoxyribonucleic acid structure and function.
复制标题
蛋白质抗生素对脱氧核糖核酸结构和功能的作用对比。
DOI：
10.1021/bi00590a015
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发表时间：
1979
期刊：
影响因子：
2.9
通讯作者：
T. Samy
中科院分区：
文献类型：
--
作者：
L. Kappen;I. Goldberg;T. Samy
比较了新癌菌素（NCS）、巨霉素（MCR）和与MCR密切相关的金霉素（AUR）在体内外对脱氧核糖核酸（DNA）的作用。NCS，显着刺激的2-巯基乙醇，是更积极地诱导超螺旋pMB 9和线性双链体λ DNA的链断裂比AUR，这是轻微抑制2-巯基乙醇。纯化的MCR，即使在非常高的水平下，也不产生任何显著量的DNA底物切割。2-丙醇刺激NCS的活性，但抑制AUR的活性。另一方面，抗氧化剂α-生育酚强烈抑制两种药物对DNA的破坏。嵌入药物溴化乙锭、柔红霉素、原黄素和放线菌素D在低浓度下抑制AUR引起的DNA断裂。将NCS活性抑制至相当水平所需的嵌入剂水平比AUR高约10倍。虽然MCR几乎没有体外DNA切割活性，但它与AUR和NCS一样具有细胞毒性，如通过在HeLa细胞中抑制DNA合成和诱导DNA链断裂所测量的。
The protein antibiotics neocarzinostain (NCS), macromomycin (MCR), and auromomycin (AUR), which is closely related to MCR, have been compared for their in vitro and in vivo actions on deoxyribonucleic acid (DNA). NCS, markedly stimulated by 2-mercaptoethanol, is much more active in inducing strand scissions in superhelical pMB9 and linear duplex lambda DNA than AUR, which is slightly inhibited by 2-mercaptoethanol. Purified MCR, even at very high levels, does not give any significant amount of cutting with either DNA substrate. 2-Propanol stimulates the activity of NCS but inhibits that of AUR. On the other hand, the antioxidant alpha-tocopherol strongly inhibits DNA breakage by both drugs. The intercalating drugs ethidium bromide, daunorubicin, proflavin, and actinomycin D at low concentrations inhibit DNA scission by AUR. The levels of intercalators required to inhibit NCS activity to comparable levels are about 10 times higher than those for AUR. Although MCR has virtually no in vitro DNA cutting activity, it is, like AUR and NCS, cytotoxic, as measured by the inhibition of DNA synthesis and induction of DNA strand breakage in HeLa cells.