Micropatterned stretching system for the investigation of mechanical tension on neural stem cells behavior
Micropatterned stretching system for the investigation of mechanical tension on neural stem cells behavior
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DOI：
10.1016/j.nano.2012.07.008
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发表时间：
2013-04-01
影响因子：
5.4
通讯作者：
Wang, Tzu-Wei
中科院分区：
文献类型：
--
作者：
Chang, Yu-Ju;Tsai, Cho-Jen;Wang, Tzu-Wei
本研究结合光刻技术和微流控技术，开发了一种可行可靠的拉伸平台，研究定向拉伸力和引导微通道对神经干细胞（NSC）行为的影响。通过不同的拉伸模式和培养条件来研究NSCs在微图案基质上的机械反应，并验证张力对NSCs成熟、轴突发芽、神经突生长和取向的影响。结果发现，在拉伸过程中，神经突延伸和轴突延伸明显增强，神经突向平行方向定向。在拉伸条件下，机械张力明显影响神经干细胞向神经元细胞分化。与平行微纹通道和垂直微纹通道相比，神经元成熟度也有显著差异。提示机械张力不仅可以引导神经突的取向和方向，还可以促进其伸长长度，触发神经干细胞分化为成熟的神经细胞。来自临床编辑：这组研究人员报告了一种可行且可靠的拉伸平台，结合光刻和微流体技术来研究定向张力和引导微通道对神经干细胞行为的影响。结果表明，神经突延伸和轴突延伸可以显著增强，神经元成熟度也可以提高。(C) 2013爱思唯尔公司版权所有。
In this study, we developed a feasible and reliable stretching platform combined with photolithography and microfluidic techniques to investigate the effect of directional tensile force and guiding microchannel on neural stem cell (NSC) behavior. Different stretching modes and culture conditions were conducted to investigate the mechanoresponse of NSCs on micropatterned substrate and to verify the effects of tension on NSCs maturation, axon sprouting, neurite outgrowth and orientation. From the results, we found that neurite extension and axon elongation were significantly enhanced and neurites were more directional orientated to parallel direction as stretching was experienced. The mechanical tension apparently influenced NSCs differentiation toward neuronal cells under stretching condition. The neuronal maturity also showed a significant difference when compared with parallel and vertical micropatterned channels. It is suggested that mechanical tension not only can guide neurites orientation and direction, but also promote their elongation length and trigger neural stem cells differentiation into mature neuronal cells.From the Clinical Editor: This group of investigators report the development of a feasible and reliable stretching platform combined with photolithography and microfluidic techniques to investigate the effects of directional tensile force and guiding microchannel on neural stem cell behavior. They demonstrate that neurite extension and axon elongation could be significantly enhanced, and neuronal maturity can also be improved. (C) 2013 Elsevier Inc. All rights reserved.