In vitro release of grape-seed polyphenols encapsulated from uncoated and chitosan-coated liposomes
In vitro release of grape-seed polyphenols encapsulated from uncoated and chitosan-coated liposomes
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葡萄籽多酚脂质体的体外释放研究
DOI：
10.1016/j.foodres.2016.02.010
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发表时间：
2016-10-01
影响因子：
8.1
通讯作者：
Weiss, Jochen
中科院分区：
文献类型：
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作者：
Gibis, Monika;Ruedt, Chiara;Weiss, Jochen
葡萄籽提取物(GSE)是多酚的丰富来源，通过高压均质(22,500磅/平方英寸)将其整合到脂质体(1.1%w/w大豆卵磷脂)中。采用壳聚糖包衣(1%w/w)可获得更稳定的脂质体。对所有脂质体的理化性质(Zeta电位、平均粒径)进行了分析。通过测定透析液(醋酸盐缓冲液，pH 3.8+/-0.1，25 mM)的总酚含量，考察了不同脂质体对GSE多酚类药物的体外释放。用不同的动力学模型描述了脂质体中多酚类药物的释放。Z-平均粒径随GSE和壳聚糖包衣的加入而增大。含有GSE的壳聚糖包衣脂质体的粒径大于不含GSE的包衣脂质体。Zeta电位由未包被脂质体的-38 mV变为包衣脂质体的+65 mV。包衣前后包封率分别为88.2+/-4.7%和99.5+/-2.3%。释药速率随时间的延长而逐渐增加。GSE多酚的体外释放符合指数方程(一阶q(T)=a.(1-exp(-k.T)。包衣脂质体的释放量明显低于未包衣脂质体。24 h后未包衣脂质体的释放度为0.55，包衣脂质体的释放度为0.24。这项研究表明，壳聚糖包衣可以减少脂质体中生物活性物质的释放，从而使包衣脂质体能够控制释放GSE多酚在水基食品中的应用。(C)2016爱思唯尔有限公司。保留所有权利。
Grape-seed extract (GSE), a rich source for polyphenols, was incorporated into liposomes (1.1% w/w soy lecithin) using high-pressure homogenization (22,500 psi). A chitosan coating (1% w/w) was used to obtain more stable liposomes. Physiochemical properties (zeta-potential, mean particle size) of all liposomes were analyzed. In vitro release of GSE-polyphenols from various liposomes was investigated by measuring the total phenolic content of the dialysate (acetate buffer, pH 3.8 +/- 0.1, 25 mM) over time. Diverse kinetic models were used to describe the release of the polyphenols incorporated from liposomes. Z-average particle diameters increased with the incorporation of GSE and chitosan coating. Chitosan-coated liposomes containing GSE had larger particle sizes than coated liposomes without GSE. The zeta-potential changed from -38 mV in uncoated liposomes to +65 mV in coated liposomes. Entrapment efficiency for uncoated and coated liposomes was 88.2 +/- 4.7% and 99.5 +/- 2.3%, respectively. The release rate increased gradually by increasing time. In vitro release of GSE polyphenols from both uncoated and coated liposomes followed an exponential equation (first order Q(t) = a.(1 - exp(-k . t))). The release from coated liposomes was much lower than uncoated liposomes. The release rate after 24 h from uncoated liposomes was 0.55 and from coated liposomes was 0.24. This study indicates that the release of bioactive compounds from liposomes can be reduced by coating with chitosan, allowing an application of coated liposomes with a controlled release of GSE polyphenols in water-based foods. (C) 2016 Elsevier Ltd. All rights reserved.