DIGITAL IMAGE-ANALYSIS OF GROWTH AND STARVATION RESPONSES OF A SURFACE-COLONIZING ACINETOBACTER SP
DIGITAL IMAGE-ANALYSIS OF GROWTH AND STARVATION RESPONSES OF A SURFACE-COLONIZING ACINETOBACTER SP
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DOI：
10.1128/jb.177.4.907-915.1995
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发表时间：
1995-02-01
影响因子：
3.2
通讯作者：
COSTERTON, JW
中科院分区：
文献类型：
--
作者：
JAMES, GA;KORBER, DR;COSTERTON, JW
采用连续流载玻片培养、相衬显微镜、扫描共聚焦激光显微镜和计算机图像分析等方法，观察了不同营养条件下不动杆菌的表面生长情况。用高营养培养基冲洗附着的球虫静止期不动杆菌细胞，使其从球虫形态向杆菌形态转变，数字图像分析显示这种转变是双相的。在第一阶段，细胞的长度和宽度都增加了。细胞宽度在II期保持不变，细胞长度和细胞面积均以大于I期的速率增加，在低营养培养基中，细胞能够生长和分裂，没有形态转变。用饥饿培养基冲洗杆状细胞后，杆状细胞通过还原分裂恢复为球菌。这导致细胞面积（生物量）随着细胞数量的增加而保持不变。此外，细胞形态的变化还伴有变化；在细胞附着的稳定性方面，球虫细胞在第一阶段保持着牢固的附着，在高营养培养基中过渡后，芽孢杆菌细胞表现出脱离、短暂附着和漂移行为，导致定植格局扩散，而在低营养培养基中冲洗的细胞仍然牢固地附着在表面，最终形成紧密排列的微菌落。据推测，球虫和杆菌不动杆菌的形态和相关行为代表了在低营养条件下（球虫形态型）的附着和定植或在高营养条件下（杆菌形态型）的分散的特殊生理适应。
Surface growth of an Acinetobacter sp. cultivated under several nutrient regimens was examined by using continuous-flow slide culture, phase-contrast microscopy, scanning confocal laser microscopy, and computer image analysis, Irrigation of attached coccoid stationary-phase Acinetobacter sp. cells with high-nutrient medium resulted in a transition from coccoid to bacillar morphology, Digital image analysis revealed that this transition was biphasic. During phase I, both the length and the width of cells increased. In contrast, cell width remained constant during phase II, while both cell length and cell area increased at a rate greater than in phase I, Cells were capable of growth and division without morphological transition when irrigated with a low-nutrient medium. Rod-shaped cells reverted to cocci by reduction-division when irrigated with starvation medium. This resulted in conservation of cell area (biomass) with an increase in cell number. In addition, the changes in cell morphology were accompanied by changes; in the stability of cell attachment, During phase I, coccoid cells remained firmly attached, Following transition in high-nutrient medium, bacillar cells displayed detachment, transient attachment, and drifting behaviors, resulting in a spreading colonization pattern, In contrast, cells irrigated with a low-nutrient medium remained firmly attached to the surface and eventually formed tightly packed microcolonies. It is hypothesized that the coccoid and bacillar Acinetobacter sp. morphotypes and associated behavior represent specialized physiological adaptations for attachment and colonization in low-nutrient systems (coccoid morphotype) or dispersion under high-nutrient conditions (bacillar morphotype).