Molecular markers for the self-compatible S4′-haplotype, a pollen-part mutant in sweet cherry (Prunus avium L.)
Molecular markers for the self-compatible S4′-haplotype, a pollen-part mutant in sweet cherry (Prunus avium L.)
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DOI：
10.21273/jashs.129.5.0724
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发表时间：
2004-09-01
影响因子：
1.9
通讯作者：
Tao, R
中科院分区：
文献类型：
--
作者：
Ikeda, K;Watari, A;Tao, R
S-4' 是甜樱桃 (Prunus avium L.) 的花粉部分突变体，广泛用于开发自交亲和品种。已知S-4'-单倍型具有功能性花柱成分和非功能性花粉成分。甜樱桃中花粉反应特异性所必需的花粉成分被认为是 S 单倍型特异性 F-box 蛋白基因，称为 SFB。这项研究描述了两种分子标记，它们利用突变等位基因中的四个碱基对缺失来区分 SFB4 和 SFB4'。所得聚合酶链反应 (PCR) 产物可以直接在聚丙烯酰胺凝胶上分离，也可以进行限制性酶消化，并且可以在琼脂糖凝胶上观察不同大小的产物。后一种技术利用在 SFB4' 等位基因的 PCR 产物中创建的限制性位点，而不是 SFB4 等位基因。由于引物组产生了差异性限制位点，因此这些引物组被称为 dCAPS（衍生的切割扩增多态性序列）标记。这些分子测定可用于验证 S-4 单倍型赋予的自身亲和性。
S-4' is a pollen-part mutant in sweet cherry (Prunus avium L.) that is extensively used to develop self-compatible cultivars. The S-4'-haplotype is known to have a functional stylar component and a nonfunctional pollen component. The pollen component in sweet cherry necessary for the specificity of the pollen reaction is believed to be an S-haplotype specific F-box protein gene, called SFB. This study describes two molecular markers that distinguish between SFB4 and SFB4' by taking advantage of a four base pair deletion in the mutant allele. The resulting polymerase chain reaction (PCR) products can either be separated directly on a polyacrylamide gel or they can be subjected to restriction enzyme digestion and the different sized products can be visualized on an agarose gel. The latter technique utilizes restriction sites created in the PCR products from the SFB4' allele, but not the SFB4 allele. Because the primer sets created differential restriction sites, these primer sets were termed dCAPS (derived cleaved amplified polymorphism sequence) markers. These molecular assays can be used to verify self-compatibility conferred by the S-4-haplotype.