Protection of DNA molecules of cultured mammalian cells from radiation-induced single-strand scissions by various alcohols and SH compounds.
Protection of DNA molecules of cultured mammalian cells from radiation-induced single-strand scissions by various alcohols and SH compounds.
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DOI：
10.1080/09553007214550401
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
R. Roots;S. Okada
中科院分区：
文献类型：
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作者：
R. Roots;S. Okada
摘要 添加醇（甲醇、乙醇、叔丁醇、乙二醇和甘油）、SH 化合物（半胱胺、半胱氨酸和巯基乙醇）和胱胺可以保护哺乳动物细胞的 DNA 分子免受辐射引起的单链断裂。这些清除剂提供的保护随着其浓度的增加而增加，但始终仅达到某个最大值。所有醇和胱胺的最大保护都是相同的，但所有 SH 化合物都有另一个最大保护。因此，辐射引起的单链断裂的程度可以分为可保护的和不可保护的部分。在可保护的部分中，半最大保护的清除剂浓度与OH自由基与清除剂反应的反应速率常数之间存在线性关系。 H 和 eaq− 的速率常数没有证明这种线性关系。这使我们得出以下建议：（1）可保护的部分主要是......
SummaryThe addition of alcohols (methanol, ethanol, t-butanol, ethylene glycol, and glycerol), SH compounds (cysteamine, cysteine and mercaptoethanol) and cystamine protected DNA molecules of mammalian cells from radiation-induced single-strand scissions. The protection afforded with these scavengers increased as their concentrations were increased, but always only up to a certain maximum. The maximum protection was the same for all the alcohols and cystamine, but another maximum was found for all the SH compounds. The extent of radiation-induced single-strand breaks can therefore be grouped into protectable and non-protectable fractions.In the protectable fraction, there is a linear relationship between the scavenger concentrations for half maximum protection and the reaction rate constants for the OH radical reacting with the scavengers. No such linear relationship was demonstrated with rate constants of H and eaq−. This leads us to the following suggestions: (1) that the protectable fraction is mostly ...