Isolation and characterization of two hydroxyproline-containing glycoproteins from normal animal lung lavage and lamellar bodies.
Isolation and characterization of two hydroxyproline-containing glycoproteins from normal animal lung lavage and lamellar bodies.
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从正常动物肺灌洗液和板层体中分离和表征两种含羟脯氨酸的糖蛋白。
DOI：
10.1172/jci108020
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
W. Lynn
中科院分区：
文献类型：
--
作者：
S. N. Bhattacharyya;M. Passero;R. Diaugustine;W. Lynn
两个糖肽，目前在颗粒材料获得肺灌洗正常兔，分离和表征。同样的两种糖肽存在于兔肺板层体的制备中。通过十二烷基硫酸钠-丙烯酰胺凝胶电泳，发现这两种糖肽的估计分子量分别为62，000和36，000，并且发现两者都含有羟脯氨酸和相对较高量的甘氨酸（分别为11%和15%）。两种糖肽的碳水化合物分析证明存在葡糖胺、唾液酸、甘露糖、岩藻糖和半乳糖。从肺泡蛋白沉积症患者的肺中分离出的层积物中发现了具有相同分子量、氨基酸和碳水化合物组成的相似糖肽。这些数据表明，这两种胶原样糖肽是包括人类在内的许多哺乳动物肺泡内的主要蛋白。
Two glycopeptides, present in particulate material obtained by pulmonary lavage from normal rabbits, were isolated and characterized. The same two glycopeptides were present in preparations of lamellar bodies from rabbit lung. The estimated molecular weights of the two glycopeptides by sodium dodecyl sulfate-acrylamide gel electrophoresis were found to be 62,000 and 36,000 and both were found to contain hydroxyproline and relatively high amount of glycine (11 and 15 per cent, respectively). Carbohydrate analysis of the two glycopeptides demonstrated the presence of glucosamine, sialic acid, mannose, Fucose, and galactose. Similar glycopeptides of the same molecular weights, and amino acid and carbohydrate compositions have been found in layage mateial isolated from lungs of patients with alveolar proteinosis. The data indicate that thses two collagen-like glycopeptides are major intra-alveolar proteins in many mammals, including humans.