HSP70 induction during exercise and heat stress in rats: role of internal temperature.
HSP70 induction during exercise and heat stress in rats: role of internal temperature.
复制标题
DOI：
10.1249/00005768-199405001-00755
复制
发表时间：
1994-05
期刊：
影响因子：
--
通讯作者：
K. Kregel;R. Skidmore;J. Gutierrez;V. Guerriero
中科院分区：
文献类型：
--
作者：
K. Kregel;R. Skidmore;J. Gutierrez;V. Guerriero
本研究的目的是确定是否积累的72-kDa热休克蛋白（HSP 70）升高，在响应延长回合的次极量运动，其中结肠温度（Tco）保持在控制水平。将Sprague-Dawley大鼠随机分配到四个测试组之一中[每组n = 8;包括每种条件的环境温度（Ta）]：（凉爽/休息; Ta = 24摄氏度）; 2）凉爽和运动（冷却/运动; Ta = 14摄氏度）; 3）非劳力加热（加热/休息; Ta = 42摄氏度）; 4）加热和运动（加热/运动; Ta = 32摄氏度）。所有干预的持续时间约为60分钟。运动回合由以17 m/min和0%等级跑步机跑步组成，而热/休息和热/运动实验由热暴露组成，当Tco达到41 ℃时终止。所有四组的基线Tco相似。在凉爽/休息和凉爽/运动组中，最终Tco与基线值没有差异，这两组之间也没有差异。在加热/休息和加热/运动组中，加热速率相似。在试验完成后30分钟，从左后肢的腓肠肌、比目鱼肌和趾长伸肌（EDL）肌肉和左心室获得组织样品。HSP 70特异性的酶联免疫吸附测定用于直接定量组织中HSP 70的绝对水平。加热和运动对腓肠肌、比目鱼肌和左心室HSP 70水平均有显著的主效应（P < 0.05）。（250字处删节）
The purpose of this study was to determine if the accumulation of the 72-kDa heat shock protein (HSP70) is elevated in response to a prolonged bout of submaximal exercise in which colonic temperature (Tco) remained at control levels. Sprague-Dawley rats were randomly assigned to one of four testing groups [n = 8 per group; ambient temperatures (Ta) for each condition are included]: 1) control (cool/rest; Ta = 24 degrees C); 2) cool and exercise (cool/exercise; Ta = 14 degrees C); 3) nonexertional heating (heat/rest; Ta = 42 degrees C); 4) heat and exercise (heat/exercise; Ta = 32 degrees C). All interventions were approximately 60 min in duration. An exercise bout consisted of treadmill running at 17 m/min and 0% grade, while the heat/rest and heat/exercise experiments consisted of heat exposure that was terminated when Tco reached 41 degrees C. Baseline Tco was similar for all four groups. In the cool/rest and cool/exercise groups, final Tco was not different from the baseline values, nor was it different between these two groups. In the heat/rest and heat/exercise groups, heating rates were similar. Tissue samples were obtained from the gastrocnemius, soleus, and extensor digitorum longus (EDL) muscles of the left hindlimb and the left ventricle 30 min after a trial was completed. An enzyme-linked immunosorbent assay specific for HSP70 was used to directly quantitate absolute levels of HSP70 in tissues. There were significant main effects of both heating and exercise for HSP70 levels in the gastrocnemius, soleus, and left ventricle (P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)