Host RNA polymerase requirements for transcription of the human hepatitis delta virus genome.
Host RNA polymerase requirements for transcription of the human hepatitis delta virus genome.
复制标题
人类丁型肝炎病毒基因组转录所需的宿主 RNA 聚合酶。
DOI：
10.1128/jvi.75.21.10161-10169.2001
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发表时间：
2001
影响因子：
5.4
通讯作者：
Taylor,J
中科院分区：
文献类型：
--
作者：
Moraleda,G;Taylor,J
丁型肝炎病毒 (HDV) 基因组的复制需要使用宿主聚合酶进行 RNA 指导的 RNA 合成。本手稿回顾了相关已发表的证据。它还提供了两项新研究，两项研究均利用瞬时转染的 Huh7 细胞进行 HDV RNA 指导的 RNA 合成。第一项研究中，将 RNA 转录抑制剂添加到转染细胞中，持续 1 至 2 天，然后分析对加工后的单位长度基因组 HDV RNA 积累的影响。对于第二项研究，转染后 6 天分离细胞核，然后使用体外径流转录来测定 RNA 转录抑制剂的效果。总体而言，数据支持这样的解释：HDV 转录不需要宿主聚合酶 I 或 III（pol I 或 III），但至少主要涉及类似于 pol II 的酶。
Replication of the genome of hepatitis delta virus (HDV) requires RNA-directed RNA synthesis using a host polymerase(s). This manuscript reviews the relevant published evidence. It also provides two new studies, both of which made use of transiently transfected Huh7 cells undergoing HDV RNA-directed RNA synthesis. For the first study, RNA transcription inhibitors were added to the transfected cells for periods of 1 to 2 days, after which assays of the effects on the accumulation of processed unit-length genomic HDV RNA were performed. For the second study, nuclei were isolated at 6 days after transfection, and then in vitro runoff transcription was used to assay the effects of RNA transcription inhibitors. Overall, the data support the interpretation that HDV transcription does not require host polymerase I or III (pol I or III) but at least primarily involves an enzyme resembling pol II.