Formation of Stomach Tissue by Organoid Culture Using Mouse Embryonic Stem Cells.
Formation of Stomach Tissue by Organoid Culture Using Mouse Embryonic Stem Cells.
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DOI：
10.1007/978-1-4939-6949-4_16
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发表时间：
2017
影响因子：
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通讯作者：
Taka-aki K. Noguchi;A. Kurisaki
中科院分区：
文献类型：
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作者：
Taka-aki K. Noguchi;A. Kurisaki
在本章中，我们描述了一种从小鼠胚胎干细胞（ES）诱导胃类器官的方法。我们采用胚状体诱导法诱导胃原始上皮细胞分化，并在Matrigel中进行三维培养。分化的类器官包含胃体特异性和胃窦特异性的成熟胃组织细胞。该协议可能是有用的各种研究在发育生物学和疾病建模的胃。
In this chapter, we describe a method for the induction of stomach organoids from mouse embryonic stem (ES) cells. We used an embryoid body-based differentiation method to induce gastric primordial epithelium covered with mesenchyme and further differentiate it in Matrigel by 3D culture. The differentiated organoid contains both corpus- and antrum-specific mature gastric tissue cells. This protocol may be useful for a variety of studies in developmental biology and disease modeling of the stomach.