Combined quick freezing, freeze‐drying, and embedding tissue at low temperature and in low viscosity resins
Combined quick freezing, freeze‐drying, and embedding tissue at low temperature and in low viscosity resins
复制标题
在低温和低粘度树脂中结合快速冷冻、冷冻干燥和包埋组织
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
Jutta Brass
中科院分区：
文献类型：
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作者：
R. Chiovetti;L. McGuffee;S. Little;E. Wheeler‐clark;Jutta Brass
The techniques of quick freezing and freeze-drying provide an alternative to the more classical methodologies of chemical fixation and dehydration with organic solvents. It is possible to embed freeze-dried tissue in low viscosity resins, either at room temperature or at subzero temperatures in Spurr's resin or Lowicryl K4M, respectively. The choice of embedding medium affords additional flexibility in postdrying and embedding conditions, since Spurr's resin allows vapor fixation with osmium tetroxide and thermal polymerization. Osmium tetroxide is not recommended for Lowicryl resins, but these media permit polymerization at subzero temperatures with ultraviolet light. Both resins have unique advantages that may be utilized, depending upon the purpose of the embedding.
In this paper, we discuss the details of preparing smooth muscle, from rabbit renal artery, by quick freezing and freeze-drying, as well as methods for the embedding of the freeze-dried tissue in both Spurr's resin and Lowicryl K4M. Although we have previously reported the ultrastructure of smooth muscle embedded in Spurr's low viscosity resin, the combination of freeze-drying and infiltration in Lowicryl K4M represents a new approach that allows the elimination of chemical fixation, dehydration with organic solvents, and heat polymerization of the embedding medium.
DOI：
10.1177/30.2.7037935
发表时间：
1982-02
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
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作者：
R. Dudek;G. Childs;A. Boyne
通讯作者：
R. Dudek;G. Childs;A. Boyne