The usefulness of p63 as a marker of breast myoepithelial cells.
The usefulness of p63 as a marker of breast myoepithelial cells.
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发表时间：
2003-11
期刊：
影响因子：
2.3
通讯作者：
A. Batistatou;D. Stefanou;E. Arkoumani;N. Agnantis
中科院分区：
文献类型：
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作者：
A. Batistatou;D. Stefanou;E. Arkoumani;N. Agnantis
肌上皮细胞外层的鉴定是乳腺病变鉴别诊断的一个有价值的线索。肌上皮细胞通常可以用标准的苏木精-伊红染色来识别，然而在病理学实践中，人们会遇到困难的情况，特别是在核心活检标本中。肌上皮细胞的免疫组化检测有几种已报道的标记物。平滑肌特异性蛋白质，如平滑肌肌动蛋白、平滑肌肌球蛋白重链、钙蛋白和h-钙调素用于突出肌上皮。平滑肌肌动蛋白是最常用的，并已被确定为一个特定的和敏感的标记物。然而，有时染色不能解释，因为肌动蛋白染色基质成纤维细胞和血管平滑肌细胞以及。S100蛋白和特异性细胞角蛋白（角蛋白5、7、14和17）也染色肌上皮细胞，但染色不是特异性的，并且不是最佳敏感性的。Maspin和CD 10是相对较新且有前途的标记物。核蛋白p63在最近的报道中引起了广泛的关注。p63-阳性肌上皮细胞已被证明包围良性上皮病变，并在原位癌中形成围绕上皮细胞的一致但不连续的边缘。浸润性癌中未见染色。p63免疫染色是细胞核的，因此即使在细胞学制备中也很容易识别。由于基质成纤维细胞和血管平滑肌细胞均未染色，因此也具有高度特异性。总之，p63似乎是一种敏感和特异性的肌上皮标志物，并可包括在免疫组化小组，旨在确定有问题的乳腺病变的肌上皮细胞。
The identification of an outer layer of myoepithelial cells is a valuable clue in the differential diagnosis of breast lesions. Myoepithelial cells can usually be appreciated with standard hematoxylin-eosin stains, however in pathology practice one encounters difficult cases, particularly in core biopsy specimens. There are several reported markers for the immunohistochemical detection of myoepithelial cells. Smooth muscle specific proteins, such as smooth muscle actin, smooth muscle myosin heavy chain, calponin and h-caldesmone are utilized to highlight myoepithelium. Smooth muscle actin is the most commonly used and has been established as a specific and sensitive marker. However, sometimes the staining can not be interpreted, since actin stains stromal fibroblasts and vascular smooth muscle cells as well. S100 protein and specific cytokeratins (keratins 5,7,14 and 17) also stain myoepithelial cells, but the staining is not specific and is not optimally sensitive. Maspin and CD10 are relatively new and promising markers. The nuclear protein p63 has attracted much attention in recent reports. p63-positive myoepithelial cells have been shown to surround benign epithelial lesions and form a consistent, although discontinuous, rim around epithelial cells in carcinomas in situ. No staining has been noted in infiltrative carcinomas. p63-immunostaining is nuclear and so it is easily appreciated, even in cytologic preparations. It is also highly specific since neither stromal fibroblasts nor vascular smooth muscle cells are stained. In conclusion, it appears that p63 is a sensitive and specific myoepithelial marker and may be included in immunohistochemical panels aiming at identifying myoepithelial cells in problematic breast lesions.