Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study
Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study
复制标题
海参夏眠机制的认识：基于TMT的蛋白质组学研究的见解
DOI：
10.1016/j.cbd.2016.06.005
复制
发表时间：
2016-09-01
影响因子：
3
通讯作者：
Wang, Tianming
中科院分区：
文献类型：
--
作者：
Chen, Muyan;Li, Xingke;Wang, Tianming
海洋无脊椎动物夏眠是一种独特的策略，夏季生存，以应对炎热的海洋条件。仿刺参Apostichopus sp.是研究环境诱导的夏眠的理想模式动物。在本研究中，我们使用串联质量标签（TMT）耦合LC-MS/MS方法来鉴定和定量的全球蛋白质组表达谱在夏眠觉醒周期的A。- 是的从海参肠中共鉴定出3920个蛋白质。其中630个蛋白质在非夏眠（活跃）、深夏眠（至少连续夏眠15天）和夏眠后觉醒（重新运动和进食）3种条件下的差异表达显著。深夏眠的海参显示出显著的差异表达蛋白（与非夏眠对照相比，143个上调和267个下调蛋白）。这些差异表达的蛋白质表明，蛋白质和磷脂可能是主要的燃料来源，在低代谢和碳水化合物代谢的普遍衰减，观察在深夏。差异表达的蛋白质还提供了第一个全球性的图片，在蛋白质合成，蛋白质折叠，DNA结合，细胞凋亡，细胞运输和信号转导，和细胞骨架蛋白在海参深夏眠期间的转变。从夏眠与深夏眠的唤醒比较，揭示了发生在夏眠的大多数蛋白质的变化一般逆转。Western blot检测进一步证实了HSP 70在夏眠中表达上调，而甲基转移酶样蛋白7A-like表达下调。我们的研究结果表明，有大量的蛋白质转录后调控在海参夏眠觉醒周期。(C)2016 Elsevier Inc. All rights reserved.
Marine invertebrate aestivation is a unique strategy for summer survival in response to hot marine conditions. The sea cucumber, Apostichopus japonicus, is an excellent model marine invertebrate for studies of environmentally-induced aestivation. In the present study, we used a tandem mass tag (TMT)-coupled LC-MS/MS approach to identify and quantify the global proteome expression profile over the aestivation-arousal cycle of A. japonicus. A total of 3920 proteins were identified from the intestine of sea cucumber. Among them, 630 proteins showed significant differential expression when comparing three conditions of sea cucumbers: non aestivating (active), deep-aestivation (at least 15 days of continuous aestivation), and arousal after aestivation (renewed moving and feeding). Sea cucumbers in deep aestivation showed substantial differentially expressed proteins (143 up-regulated and 267 down-regulated proteins compared with non-aestivating controls). These differentially expressed proteins suggested that protein and phospholipid probably are major fuel sources during hypometabolism and a general attenuation of carbohydrate metabolism was observed during deep aestivation. Differentially expressed proteins also provided the first global picture of a shift in protein synthesis, protein folding, DNA binding, apoptosis, cellular transport and signaling, and cytoskeletal proteins during deep aestivation in sea cucumbers. A comparison of arousal from aestivation with deep aestivation, revealed a general reversal of the changes that occurred in aestivation for most proteins. Western blot detection further validated the significant up-regulation of HSP70 and down-regulation of methyltransferase-like protein 7A-like in deep-aestivation. Our results suggest that there is substantial post-transcriptional regulation of proteins during the aestivation arousal cycle in sea cucumbers. (C) 2016 Elsevier Inc. All rights reserved.