Molecular Profiling of Stomatal Meristemoids Reveals New Component of Asymmetric Cell Division and Commonalities among Stem Cell Populations in Arabidopsis
Molecular Profiling of Stomatal Meristemoids Reveals New Component of Asymmetric Cell Division and Commonalities among Stem Cell Populations in Arabidopsis
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DOI：
10.1105/tpc.111.088583
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发表时间：
2011-09-01
期刊：
影响因子：
11.6
通讯作者：
Torii, Keiko U.
中科院分区：
文献类型：
--
作者：
Pillitteri, Lynn Jo;Peterson, Kylee M.;Torii, Keiko U.
干细胞的维持和分化之间的平衡是发育生物学的中心问题。拟南芥气孔的发育始于从头不对称分裂，产生分生组织，增殖具有干细胞样特性的前体细胞。分生组织的瞬时性和非同步性使得研究其分子特征变得困难。由于SPEECHLESS、MUTE和SCREAM中的功能丧失或获得突变导致的气孔分化突变体的合成组合分别产生具有主要由铺面细胞、分生组织或气孔组成的表皮的幼苗。通过转录组分析，我们定义和特征的分生组织样的分子签名。报告基因定位的分生组织富集蛋白质的研究揭示了以前没有与气孔发育相关的途径。我们确定了一种新的蛋白质，POLAR，并通过延时实时成像表明，它具有短暂的极性定位和分离不均匀分生组织不对称分裂。极性的极性定位需要气孔线不对称的打破。比较生物信息学分析的转录谱的分生组织与芽和根顶端分生组织突出细胞分裂素信号和ERECTA家族受体样激酶在干细胞群体的广泛调节。我们的工作揭示了气孔干细胞的分子组成，并阐明了植物干细胞群体之间的一个共同主题。
The balance between maintenance and differentiation of stem cells is a central question in developmental biology. Development of stomata in Arabidopsis thaliana begins with de novo asymmetric divisions producing meristemoids, proliferating precursor cells with stem cell-like properties. The transient and asynchronous nature of the meristemoid has made it difficult to study its molecular characteristics. Synthetic combination of stomatal differentiation mutants due to loss-or gain-of-function mutations in SPEECHLESS, MUTE, and SCREAM create seedlings with an epidermis overwhelmingly composed of pavement cells, meristemoids, or stomata, respectively. Through transcriptome analysis, we define and characterize the molecular signatures of meristemoids. The reporter localization studies of meristemoid-enriched proteins reveals pathways not previously associated with stomatal development. We identified a novel protein, POLAR, and demonstrate through time-lapse live imaging that it exhibits transient polar localization and segregates unevenly during meristemoid asymmetric divisions. The polar localization of POLAR requires BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE. Comparative bioinformatic analysis of the transcriptional profiles of a meristemoid with shoot and root apical meristems highlighted cytokinin signaling and the ERECTA family receptor-like kinases in the broad regulation of stem cell populations. Our work reveals molecular constituents of stomatal stem cells and illuminates a common theme among stem cell populations in plants.