Photosensitizing activity of hematoporphyrin on Staphylococcus aureus cells
Photosensitizing activity of hematoporphyrin on Staphylococcus aureus cells
复制标题
DOI：
10.1016/s0304-4165(00)00071-4
复制
发表时间：
2000-07-03
影响因子：
3
通讯作者：
Merchat, M
中科院分区：
文献类型：
--
作者：
Bertoloni, G;Lauro, FM;Merchat, M
研究了血卟啉（Hp）对两种金黄色葡萄球菌菌株的光敏作用，以确定光过程除了在细胞质膜水平上发生的损伤外，是否还诱导体内DNA损伤。所获得的结果表明，光杀伤是依赖于Hp剂量，即使这两种菌株，具有相似的Hp结合能力，表现出不同水平的光敏性。细胞质膜蛋白和DNA（染色体和plasmidial）的电泳分析表明，膜代表的主要目标的光过程，而DNA，这是在体内和体外损伤，只有在相对较长的照射时间，可能是一个次要的目标。此外，光处理导致鼠伤寒沙门氏菌试验菌株发生诱变。鉴于光动力疗法在治疗微生物感染方面的潜在用途，该信息尤其重要。(C)2000 Elsevier Science B.V.保留所有权利。
The photosensitizing action of hematoporphyrin (Hp) on two Staphylococcus aureus strains was investigated to determine if the photoprocess induces in vivo damage in DNA in addition to that occurring at the level of the cytoplasmic membrane. The results obtained demonstrate that the photokilling is dependent on the Hp dose even though the two strains, having a similar Hp-binding capacity, show different levels of photosensitivity. The electrophoretic analysis of cytoplasmic membrane proteins and DNA (chromosomal and plasmidial) suggests that the membrane represents the primary target of the photoprocess, while the DNA, that is damaged both in vivo and in vitro only at relatively long irradiation time, might be a secondary target. Moreover, the photoprocess results in mutagenesis for Salmonella typhimurium tester strains. This information is particularly important in view of the potential use of photodynamic therapy for the treatment of microbial infections. (C) 2000 Elsevier Science B.V. All rights reserved.