YRA1 autoregulation requires nuclear export and cytoplasmic Edc3p-mediated degradation of its Pre-mRNA
YRA1 autoregulation requires nuclear export and cytoplasmic Edc3p-mediated degradation of its Pre-mRNA
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DOI：
10.1016/j.molcel.2007.01.012
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发表时间：
2007-02-23
期刊：
影响因子：
16
通讯作者：
He, Feng
中科院分区：
文献类型：
--
作者：
Dong, Shuyun;Li, Chunfang;He, Feng
自动调节环通常提供对编码关键调节蛋白的特定基因的表达水平的精确控制。在这里，我们已经定义了一个途径，Yra1p，酵母mRNA输出因子，控制自己的表达。我们表明，顺式的YRA 1外显子1序列和反式的Yra 1 p序列抑制YRA 1前体mRNA剪接并使前体mRNA进入核输出。Mex67p和Crm1p共同促进YRA1前体mRNA输出，一旦进入细胞质，前体mRNA通过依赖于去帽激活剂Edc3p和YRA1内含子中的特定序列的5'至3'衰变机制降解。这些结果说明了如何在核处理，出口和降解的转录的共同步骤可以独特地结合起来，以控制特定基因的表达，并建议Edc3p介导的衰变可能有额外的调节功能，在真核细胞。
Autoregulatory loops often provide precise control of the level of expression of specific genes that encode key regulatory proteins. Here we have defined a pathway by which Yra1p, a yeast mRNA export factor, controls its own expression. We show that YRA1 exon 1 sequences in cis and Yra1p in trans inhibit YRA1 pre-mRNA splicing and commit the premRNA to nuclear export. Mex67p and Crm1p jointly promote YRA1 pre-mRNA export, and once in the cytoplasm, the pre-mRNA is degraded by a 5' to 3' decay mechanism that is dependent on the decapping activator Edc3p and on specific sequences in the YRA1 intron. These results illustrate how common steps in the nuclear processing, export, and degradation of a transcript can be uniquely combined to control the expression of a specific gene and suggest that Edc3p-mediated decay may have additional regulatory functions in eukaryotic cells.