MENAQUINONE PRODUCTION AND UTILIZATION IN GERM-FREE RATS AFTER INOCULATION WITH SPECIFIC ORGANISMS
MENAQUINONE PRODUCTION AND UTILIZATION IN GERM-FREE RATS AFTER INOCULATION WITH SPECIFIC ORGANISMS
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DOI：
10.1093/jn/117.6.1032
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发表时间：
1987-06-01
影响因子：
4.2
通讯作者：
NAKAO, H
中科院分区：
文献类型：
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作者：
KINDBERG, C;SUTTIE, JW;NAKAO, H
虽然菜籽酮是维生素K的生物活性形式，但影响它们由细菌产生或从肠道吸收的因素还不是很清楚。将无菌雄性大鼠接种四种不同的微生物菌株，测定粪便和组织中的孟喹酮浓度。在长双歧杆菌或梭状芽孢杆菌定植的大鼠的组织或粪便中没有检测到孟喹酮，这两种生物在纯培养条件下生长时还没有报告产生孟喹酮。感染寻常型杆菌的大鼠粪便中MK-10含量较高，其中MK-9和MK-11含量较高，而大肠埃希氏菌感染的大鼠粪便中MK-8含量较高，MK-7含量较少。在这些生物体的纯培养中也会产生相同的菜籽酮。在肝脏中也检测到了主要的粪便中的甲喹酮，并且在那些没有保持在防止吞噬的笼子中的大鼠的肝脏中存在更高的浓度。
Although menaquinones are biologically active forms of vitamin K, factors that influence their production by bacteria or their absorption from the gut are not well understood. Germ-free male rats were inoculated with four different strains of organisms and fecal and tissue menaquinone concentrations were determined. No menaquinones were detected in the tissues or feces of rats colonized with Bifidobacterium longum or Clostridium ramosum, two organisms that have not been reported to produce menaquinones when grown in pure cultures. Rats colonized with Bacteriodes vulgatus had high levels of fecal MK-10 with significant amounts of MK-9 and MK-11, whereas rats colonized with Escherichia coli had high levels of fecal MK-8 and small amounts of MK-7. The same menaquinones are produced in pure cultures of these organisms. The predominant fecal menaquinones were also detected in liver and were present in higher concentrations in the liver of those rats not maintained in coprophagy-preventing cages.