LEUNIG has multiple functions in gynoecium development in Arabidopsis
LEUNIG has multiple functions in gynoecium development in Arabidopsis
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DOI：
10.1002/(sici)1526-968x(200001)26:1
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发表时间：
2000-01-01
期刊：
影响因子：
1.5
通讯作者：
Huang, H
中科院分区：
文献类型：
--
作者：
Chen, CB;Wang, SP;Huang, H
拟南芥基因LEUNIG先前被发现调节花器官的特性。在这项工作中，我们描述了新分离的强leunig等位基因，leunig-101， leunig-102和leunig-103的雌蕊表型。这些强壮的突变体的雌蕊只在基部合生，在先端留下四个不合生的部分。其中两个中间的是花柱顶有柱头，两个外侧的是从瓣的突出物。leunig是由基部分生组织带形成的，表明leunig是正常先天性雌蕊融合的必要条件；leunig生长中的雌蕊内表面表皮细胞相互接触后不能融合，表明leunig是正常生殖后融合的必要条件。这些瓣膜偶尔也有柱头样组织，表明确定瓣膜身份需要LEUNIG功能。我们还分析了clavata1-4 - leunig-101、clavata1- 1 lug-101、fruit1 leunig-101和pinoid-1 leunig-101双突变体。clavata1-4 - LEUNIG -101和clavata2-1 - LEUNIG -101表现出单突变体的加性表型，表明LEUNIG和CLAVATA基因在不同的途径中起作用。相反，frufull和PINOID基因与LEUNIG相互作用，调节雌蕊发育。(C) 2000 Wiley-Liss, Inc。
The Arabidopsis gene LEUNIG was previously found to regulate floral organ identity. In this work we describe gynoecial phenotypes of newly isolated strong leunig alleles, leunig-101, leunig-102, and leunig-103. Gynoecia of these strong leunig mutants are united only at the basal part, leaving four unfused parts at the apex. Among them two medial ones are styles capped with stigmas, and two lateral ones are protrusions from valves. The gynoecium with unfused apex in leunig arises as a unit from a basal meristematic zone, suggesting that LEUNIG is required for normal congenital gynoecium fusion, the epidermal cells on growing inner surfaces of leunig gynoecium failed to fuse after they contact each other, indicating that LEUNIG is essential for the proper postgenital fusion, The epidermal cells at the very distal portion of protruded valves mimic those on wild-type styles, and those valves occasionally also have stigma-like tissues, indicating that LEUNIG function is required for the valve identity determination. We have also analyzed clavata1-4 leunig-101, clavata2-1 lug-101, fruitfull-1 leunig-101, and pinoid-1 leunig-101 double mutants. clavata1-4 leunig-101 and clavata2-1 leunig-101 exhibited additive phenotypes of single mutants, suggesting that LEUNIG and CLAVATA genes function in different pathways. In contrast, FRUITFULL and PINOID genes interact with LEUNIG to regulate gynoecium development. (C) 2000 Wiley-Liss, Inc.