RADIOIMMUNOASSAY OF HUMAN EPIDERMAL GROWTH-FACTOR - HEGF LEVELS IN HUMAN-BODY FLUIDS
RADIOIMMUNOASSAY OF HUMAN EPIDERMAL GROWTH-FACTOR - HEGF LEVELS IN HUMAN-BODY FLUIDS
复制标题
DOI：
10.1248/bpb1978.11.146
复制
发表时间：
1988-03-01
期刊：
影响因子：
--
通讯作者：
SAKAMOTO, S
中科院分区：
文献类型：
--
作者：
HAYASHI, T;SAKAMOTO, S
用基因工程技术构建的大肠杆菌合成的人表皮生长因子（hEGF）免疫家兔，获得了高效价的hEGF抗血清。该抗血清对hEGF {hEGF，hEGF [1-51]，hEGF [1-47]和[Leu 21] hEGF}具有良好的特异性，并且与其它生物肽如小鼠EGF和人血小板衍生生长因子几乎没有交叉反应性。用放射性碘标记人表皮生长因子（hEGF），用双抗体-聚乙二醇法建立了hEGF的放射免疫分析法（RIA）。用本放免分析法可成功地测定低至10 μ g/管的hEGF。人血浆或血清中hEGF的批内和批间变异系数分别为2.4-4.9%和3.7- 6.2%。用本方法测定的人体液中hEGF水平如下：尿液（11-100 ng/ml）、血清（1.0-1.8 ng/ml）、血浆（0.2-0.8 ng/ml）、精子（17-47 ng/ml）、唾液（0.9-3.0 ng/ml）和泪液（9.5-27 ng/ml）。
By immunizing rabbits with purified human epidermal growth factor (hEGF), which was synthesized by Escherichia coli constructed by a genetic engineering technique, high titer antiserum for hEGF was obtained. The antiserum has an excellent specificity to hEGFs {hEGF, hEGF [1-51], hEGF [1-47] and [Leu21] hEGF} and has almost no cross-reactivity with other biological peptides, such as mouse EGF and human platelet derived growth factor. Using radioiodinated hEGF, a radioimmunoassay (RIA) for hEGF was established by a double antibody-polyethylene glycol method. As little as 10 pg/tube of hEGF was successfully determined by the present RIA methods. The intra- and inter-assay coefficients of variation for hEGF in human plasma or serum were 2.4-4.9% and 3.7-6.2%, respectively. The hEGF levels in human body fluids determined by the present method were as follows: urine (11-100 ng/ml), serum (1.0-1.8 ng/ml), plasma (0.2-0.8 ng/ml), sperm (17-47 ng/ml), saliva (0.9-3.0 ng/ml) and tears (9.5-27 ng/ml).