Enzymatic Saccharification of Dilute Alkaline Pre-treated Microalgal (Tetraselmis suecica) Biomass for Biobutanol Production
Enzymatic Saccharification of Dilute Alkaline Pre-treated Microalgal (Tetraselmis suecica) Biomass for Biobutanol Production
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稀碱预处理微藻 (Tetraselmis suecica) 生物质的酶糖化用于生物丁醇生产
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发表时间：
2014
期刊：
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通讯作者：
S. Bhattacharya
中科院分区：
文献类型：
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作者：
M. A. Kassim;Ravichandra Potumarthi;A. Tanksale;Srikanth Chakravartula Srivatsa;S. Bhattacharya
生物质的酶解糖化用于还原糖生产是生物燃料通过生化转化生产的关键过程之一。本研究以长直链木霉为原料，利用纤维素酶对经预处理的稀氢氧化钾（KOH）预处理的水木螺生物质进行酶解糖化。首先，通过改变碱试剂浓度、反应停留时间和温度对预处理条件进行优化。利用傅里叶变换红外光谱和扫描电镜对预处理后的水芹生物量进行了表征。结果表明，预处理改变了水蛭生物量的乙酰基、羟基、结构和表面等官能团，有利于酶解糖化过程。未经处理和预处理的微藻生物量的酶解糖化比较表明，预处理的微藻可以获得更高水平的还原糖。通过改变温度、pH和酶固比（E /[S]），优化了预处理水蛭生物质的酶解糖化过程。在温度为40°C、pH为4.5、[E]/[S]为0.1的条件下，培养72 h后，经预处理的水蛭糖化，碳水化合物转化为还原糖的最高转化率为95%，相当于还原糖产量为20% （wt%）。以糖化过丁酸梭菌为生物催化剂，对经预处理的水解液进行酶解发酵制备生物丁醇。本研究结果表明，稀碱性预处理在促进微藻生物质酶解糖化和生物丁醇生产方面具有积极的应用前景。
— Enzymatic saccharification of biomass for reducing sugar production is one of the crucial processes in biofuel production through biochemical conversion. In this study, enzymatic saccharification of dilute potassium hydroxide (KOH) pre-treated Tetraselmis suecica biomass was carried out by using cellulase enzyme obtained from Trichoderma longibrachiatum . Initially, the pre-treatment conditions were optimised by changing alkali reagent concentration, retention time for reaction, and temperature. The T. suecica biomass after pre-treatment was also characterized using Fourier Transform Infrared Spectra and Scanning Electron Microscope. These analyses revealed that the functional group such as acetyl and hydroxyl groups, structure and surface of T. suecica biomass were changed through pre-treatment, which is favourable for enzymatic saccharification process. Comparison of enzymatic saccharification of untreated and pre-treated microalgal biomass indicated that higher level of reducing sugar can be obtained from pre-treated T. suecica . Enzymatic saccharification of pre-treated T. suecica biomass was optimised by changing temperature, pH, and enzyme concentration to solid ratio ([E]/[S]). Highest conversion of carbohydrate into reducing sugar of 95% amounted to reducing sugar yield of 20 (wt%) from pre-treated T. suecica was obtained from saccharification, at temperature: 40°C, pH: 4.5 and [E]/[S] of 0.1 after 72 h of incubation. Hydrolysate obtained from enzymatic saccharification of pretreated T. suecica biomass was further fermented into biobutanol using Clostridium saccharoperbutyliticum as biocatalyst. The results from this study demonstrate a positive prospect of application of dilute alkaline pre-treatment to enhance enzymatic saccharification and biobutanol production from microalgal biomass.