Effects of Quercetin on Oxidative Stress Biomarkers in Methimazole - Induced Hypothyroid Rats
Effects of Quercetin on Oxidative Stress Biomarkers in Methimazole - Induced Hypothyroid Rats
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DOI：
10.1055/s-0034-1376968
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发表时间：
2014-10-01
影响因子：
1.8
通讯作者：
Loro, V. L.
中科院分区：
文献类型：
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作者：
Santi, A.;Baldissareli, J.;Loro, V. L.
本研究的目的是评价栎素对他巴唑(MMI)诱导的甲减雄性大鼠氧化应激生物标志物的影响。以20 mg/100mlMMI灌胃1个月，诱发甲状腺功能减退。大鼠甲状腺功能减退后，分别给予10 mg/kg和25 mg/kg的QT灌胃，连续8周。雄性Wistar大鼠60只，随机分为6组(I组，对照组；II组，QT10组；III组，QT25组；IV组，甲状腺功能低下组；V组，甲状腺功能低下+QT10组；VI组，甲状腺功能低下+QT25组)。与对照组相比，甲减大鼠的肝脏、肾脏和血清中的TBARS水平显著升高，同时肝脏中的蛋白质羰基(PCO)增加，肝和肾脏组织中的ROS水平增加。QT10和QT25能有效降低血清和肾脏的TBARS水平，降低肝脏的PCO水平和肝、肾的ROS生成。MMI诱导的甲状腺功能减退也增加了大脑皮层和海马区的TBARS水平，而在服用QT25的大鼠中，这两个水平又降低了。甲减大鼠服用QT25后，肝脏和全血中超氧化物歧化酶活性降低，肝脏过氧化氢酶活性升高。肝脏和肾脏的抗坏血酸水平在补充两种浓度的槲皮素后恢复。QT10和QT25还能显著提高甲状腺功能低下大鼠肝、肾组织的总清除氧化能力。这些发现表明，MMI诱导的甲状腺功能减退症增加了氧化应激参数，给药可能对甲状腺功能减退状态下的氧化还原失衡起到有益的作用。
The objective of the present study was to evaluate the effect of quercetin on oxidative stress biomarkers in methimazole (MMI) -induced hypothyroidism male rats. Hypothyroidism was induced by administering MMI at 20 mg/100 ml in the drinking water, for 1 month. After achieved hypothyroidism, rats received orally 10 or 25 mg/kg of quercetin (QT) for 8 weeks. 60 male wistar rats were randomly divided into 6 groups (group I, control; group II, QT10; group III, QT25; group IV, hypothyroid; group V, hypothyroid + QT10; group VI, hypothyroid + QT25). Liver, kidney and serum TBARS levels significantly increased in hypothyroid rats when compared to controls, along with increased protein carbonyl (PCO) in liver and increased ROS levels in liver and kidney tissues. QT10 and QT25 were effective in decreasing TBARS levels in serum and kidney, PCO levels in liver and ROS generation in liver and kidney. MMI -induced hypothyroidism also increased TBARS levels in cerebral cortex and hippocampus that in turn were decreased in rats treated with QT25. Moreover, the administration of QT25 to hypothyroid rats resulted in decreased SOD activities in liver and whole blood and increased liver CAT activity. Liver and kidney ascorbic acid levels were restored with quercetin supplementation at both concentrations. QT10 and QT25 also significantly increased total oxidative scavenging capacity in liver and kidney tissues from hypothyroid rats. These findings suggest that MMI -induced hypothyroidism increases oxidative stress parameters and quercetin administration could exert beneficial effects against redox imbalance in hypothyroid status.