Sensitive competitive immunoassay of multiple mycotoxins with non-fouling antigen microarray
Sensitive competitive immunoassay of multiple mycotoxins with non-fouling antigen microarray
复制标题
使用无污染抗原微阵列对多种霉菌毒素进行灵敏的竞争性免疫分析
DOI：
10.1016/j.bios.2013.06.037
复制
发表时间：
2013-12-15
影响因子：
12.6
通讯作者：
Li, Chang Ming
中科院分区：
文献类型：
--
作者：
Hu, Weihua;Li, Xin;Li, Chang Ming
真菌毒素具有强致癌性和毒性，是食品安全的致命威胁，对检测方法的高灵敏度和高通量要求很高。本文以黄曲霉毒素B1（AFB 1）、赭曲霉毒素A（OTA）和玉米赤霉烯酮（ZEN）为模板，建立了一种基于聚甲基丙烯酸乙二醇酯-甲基丙烯酸缩水甘油酯（POEGMA-co-GMA）无污染聚合物刷的荧光竞争免疫分析芯片。由于POEGMA-co-GMA刷具有均匀的大蛋白负载量和对非特异性蛋白吸收的高抗性，最佳微阵列表现出三个数量级的宽动态范围和分别为4、4和3 pg mL（-1）的低检测限，这比用环氧官能化抗原微阵列获得的结果好得多，并且与常规ELISA方法相当甚至更好。本研究为食品质量和环境监测中毒素的快速筛选提供了一个强有力的高通量工具。(C)2013爱思唯尔有限公司版权所有。
Various mycotoxins with strong carcinogenesis and toxicity are fatal threats in food safety, and require highly sensitive and high-throughput detections greatly. Herein a fluorescent competitive immunoassay microarray based on a non-fouling polymer brush, poly[(ethylene glycol) methacylate-co-glycidyl methacrylatej (POEGMA-co-GMA) is explored to sensitively detect multiple mycotoxins with aflatoxin B1 (AFB1), ochratoxin A (OTA) and zearalenone (ZEN) as template targets. Due to uniformly large protein loading and high resistance to nonspecific protein absorption of the POEGMA-co-GMA brush, the optimal microarray exhibits wide dynamic ranges of three orders of magnitudes and low detection limits of 4, 4 and 3 pg mL(-1) respectively, which is much better than that obtained with an epoxy-functionalized antigen microarray, and is comparable or even better than the conventional ELISA method. This work offers a powerful high-throughput tool to fast screening of toxins in food quality and environmental monitoring. (C) 2013 Elsevier B.V. All rights reserved.