SERS Detection of Biomolecules by Highly Sensitive and Reproducible Raman-Enhancing Nanoparticle Array.
SERS Detection of Biomolecules by Highly Sensitive and Reproducible Raman-Enhancing Nanoparticle Array.
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DOI：
10.1186/s11671-017-2121-x
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发表时间：
2017-12
影响因子：
--
通讯作者：
Wang YL
中科院分区：
文献类型：
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作者：
Chan TY;Liu TY;Wang KS;Tsai KT;Chen ZX;Chang YC;Tseng YQ;Wang CH;Wang JK;Wang YL
本文描述了用于表面增强拉曼散射（Sers）基底的由银纳米颗粒（AgNPs：20-50 nm）组成的纳米阵列的制备。采用电化学电镀法在多孔阳极氧化铝（AAO）模板上制备了AgNPs，其通道间距为10 ~ 20 nm。调整银颗粒的尺寸和颗粒间间隙以获得最佳Sers信号，并通过扫描电子显微镜、原子力显微镜和拉曼光谱进行表征。当测量腺嘌呤溶液时，Sers强度的波动约为10-20%，显示出良好的可重复性Sers传感。基于SERS的腺嘌呤（A）、胸腺嘧啶（T）、胞嘧啶（C）、鸟嘌呤（G）、β-胡萝卜素和孔雀石绿（绿色）的定量检测和区分显示了纳米颗粒阵列在实际应用中的巨大潜力。腺嘌呤的检出限<1 ppb，β-胡萝卜素和孔雀石绿色的检出限<0.63 ppm。通过嵌入阳极氧化铝中的Ag纳米颗粒阵列实现的均匀且可再现的拉曼增强区分并帮助定量DNA规范核碱基（腺嘌呤、胸腺嘧啶、胞嘧啶和鸟嘌呤）。
This paper describes the preparation of nanoarrays composed of silver nanoparticles (AgNPs: 20–50 nm) for use as surface-enhanced Raman scattering (SERS) substrates. The AgNPs were grown on porous anodic aluminum oxide (AAO) templates by electrochemical plating, and the inter-channel gap of AAO channels is between 10 and 20 nm. The size and interparticle gap of silver particles were adjusted in order to achieve optimal SERS signals and characterized by scanning electron microscopy, atomic force microscopy, and Raman spectroscopy. The fluctuation of SERS intensity is about 10–20% when measuring adenine solutions, showing a great reproducible SERS sensing. The nanoparticle arrays offer a large potential for practical applications as shown by the SERS-based quantitative detection and differentiation of adenine (A), thymine (T), cytosine (C), guanine (G), β-carotene, and malachite green. The respective detection limits are <1 ppb for adenine and <0.63 ppm for β-carotene and malachite green, respectively. Uniform and reproducible Raman enhancement enabled by Ag nanoparticle array embedded in anodic aluminum oxide differentiates and helps quantify DNA canonical nucleobases (adenine, thymine, cytosine, and guanine).