STRUCTURE AND ARRANGEMENT OF THE CHOLERA-TOXIN GENES IN VIBRIO-CHOLERAE O139
STRUCTURE AND ARRANGEMENT OF THE CHOLERA-TOXIN GENES IN VIBRIO-CHOLERAE O139
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DOI：
10.1016/0378-1097(94)90194-5
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发表时间：
1994-04-01
影响因子：
2.1
通讯作者：
HOLMGREN, J
中科院分区：
文献类型：
--
作者：
LEBENS, M;HOLMGREN, J
已经确定了编码霍乱毒素的B亚单位的ctx B基因的序列，该序列与最近在整个东南亚爆发的严重霍乱有关的新的O 139血清型霍乱弧菌菌株，并发现其与El Tor生物型霍乱弧菌O 1中的ctx B基因相同。Southern杂交和PCR分析表明，所有O139血清型霍乱弧菌菌株都携带霍乱毒素基因和其他与毒力盒DNA区相关的基因，其两个位点与经典型霍乱弧菌血清型O1中鉴定的位点相同或同源，而不是El Tor生物型霍乱弧菌血清型O1中鉴定的位点，尽管O139中的这些位点可能存在于大小可变的限制性片段上。
The sequence of the ctxB gene encoding the B subunit of cholera toxin has been determined for a strain of Vibrio cholerae of the novel O139 serotype associated with recent outbreaks of severe cholera throughout South-East Asia and found to be identical to the ctxB gene in V. cholerae O1 of the El Tor biotype. Analyses by Southern hybridization and PCR showed that all strains of the O139 serotype V. cholerae tested carried cholera toxin genes and other genes associated with a virulence cassette DNA region at two loci identical or homologous to those identified in the Classical rather than the El Tor biotype of V. cholerae serotype O1 although these loci in O139 could reside on restriction fragments of variable size.