Exclusion of HIV coreceptors CXCR4, CCR5, and CCR3 from the HIV envelope.
Exclusion of HIV coreceptors CXCR4, CCR5, and CCR3 from the HIV envelope.
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将 HIV 辅助受体 CXCR4、CCR5 和 CCR3 从 HIV 包膜中排除。
DOI：
10.1089/088922299310601
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Bandres,JC
中科院分区：
文献类型：
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作者：
Lallos,LB;Laal,S;Hoxie,JA;Zolla-Pazner,S;Bandres,JC
HIV-1原代分离株和实验室毒株都将细胞衍生的分子掺入其包膜中，这取决于它们生长的宿主细胞。这种结合不是随机的，具体地说， HIV-1已经显示出选择性地对抗其主要受体CD 4结合到其表面。在这项研究中，我们观察了HIV辅助受体CXCR 4、CCR 5和CCR 3掺入细胞中的情况。 艾滋病毒信封。为此，我们在几种细胞系和PBMC中培养HIV-1原代分离株BZ 167，并用病毒结合ELISA测定所得病毒的包膜特征。而 病毒颗粒在通过不同细胞系时获得几个分子（例如，ICAM-3、LFA-1、ICAM-1或MHC II类），BZ 167从未掺入显著水平的CXCR 4、CCR 5或CCR 3 即使生长它的部分或全部细胞系表达它们，这些结果表明，HIV-1选择性地抑制这些趋化因子受体进入其包膜 分子，因为它对CD 4的掺入。
Both HIV-1 primary isolates and laboratory strains incorporate cell-derived molecules into their envelopes depending on the host cell in which they are grown. This incorporation is not random and, specifically, HIV-1 has been shown to select against the incorporation into its surface of CD4, its main receptor. In this study, we have looked at the incorporation of HIV coreceptors CXCR4, CCR5, and CCR3 into the HIV envelope. For this purpose, we grew HIV-1 primary isolate BZ167 in several cell lines and PBMCs, and the envelope profiles of the resulting viruses were determined with a virus-binding ELISA. While the virus particle gained several molecules when passed through the different cell lines (e.g., ICAM-3, LFA-1, ICAM-1, or MHC class II), BZ167 never incorporated significant levels of CXCR4, CCR5, or CCR3 into its envelope even though some or all of the cell lines in which it was grown expressed them. These results show that HIV-1 selects against the incorporation of these chemokine receptors into its envelope molecule, as it does against the incorporation of CD4.