Accessory olfactory bulb neurons are required for maintenance but not induction of V2R vomeronasal receptor gene expression in vitro
Accessory olfactory bulb neurons are required for maintenance but not induction of V2R vomeronasal receptor gene expression in vitro
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DOI：
10.1016/j.neulet.2011.05.232
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发表时间：
2011-08
影响因子：
2.5
通讯作者：
K. Muramoto;K. Hagino‐Yamagishi;K. Tonosaki;H. Kaba
中科院分区：
文献类型：
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作者：
K. Muramoto;K. Hagino‐Yamagishi;K. Tonosaki;H. Kaba
许多哺乳动物通过感觉器官犁鼻器（VNO）检测信息素。在之前的一项使用免疫印迹和免疫细胞化学分析的研究中，我们报道了与单独使用 VNO 的培养物相比，VNO 与副嗅球 (AOB) 神经元的共培养导致犁鼻感觉神经元 (VSN) 的成熟和 V2R 家族犁鼻受体的表达更高。为了进一步表征 V2R 表达，我们使用 RT-PCR 分析研究了 AOB 神经元存在或不存在时 V2R mRNA 表达的时间过程。不仅在与AOB神经元共培养3天的VNO中，而且在单独培养相同天数的VNO中都已经检测到V2R mRNA的表达。然而，在额外培养期间，单独培养的VNO中V2R mRNA的表达显着降低，尽管在共培养的VNO中V2R mRNA的表达持续表达。此外，向共培养的VNO施用2μM TTX导致V2R mRNA表达显着降低至与共培养中单独培养14天的VNO相同的水平。我们之前的工作假设是 VSN 中 V2R 的表达是通过与 AOB 神经元相互作用诱导的。然而，目前的结果表明，VSN 中的受体表达在早期发育阶段不依赖于与 AOB 神经元的相互作用，而是通过与 AOB 神经元的主动相互作用来维持。
Many mammals detect pheromones by a sensory organ, the vomeronasal organ (VNO). In a previous study using immunoblot and immunocytochemical analyses, we reported that cocultures of VNOs with accessory olfactory bulb (AOB) neurons resulted in the maturation of vomeronasal sensory neurons (VSNs) and a greater expression of V2R family vomeronasal receptors than cultures with VNO alone. To further characterize the V2R expression, we here investigated the time course of the expression of V2R mRNA in the presence or absence of AOB neurons using RT-PCR analysis. The expression of V2R mRNA was already detectable not only in the VNO cocultured with AOB neurons for 3 days in coculture but also in the VNO cultured alone for the same number of days. However, the expression of V2R mRNA in the VNO cultured alone was remarkably decreased during the additional culture period, although that in the cocultured VNO showed sustained expression. Moreover, the application of 2μM TTX to the cocultured VNO resulted in a marked decrease in the V2R mRNA expression to a level equal to that in the VNO cultured alone for 14 days in coculture. Our previous working hypothesis was that the expression of V2Rs in VSNs was induced by interacting with AOB neurons. However, the present results suggest that the receptor expression in VSNs is independent of the interaction with AOB neurons in the early developmental stage, but is maintained by the active interaction with AOB neurons.