Changes in the physicochemical properties of fish cell membranes during cellular senescence
Changes in the physicochemical properties of fish cell membranes during cellular senescence
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DOI：
10.1080/09168451.2019.1695576
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发表时间：
2019-11
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影响因子：
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通讯作者：
Akifumi Shiomi;K. Nagao;H. Kasai;Y. Hara;M. Umeda
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文献类型：
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作者：
Akifumi Shiomi;K. Nagao;H. Kasai;Y. Hara;M. Umeda
鱼类细胞系被广泛用于发育生物学、病毒学、衰老生物学和营养生理学的研究。然而，人们对它们的物理化学性质知之甚少。本文报道了淡水、溯河和海洋鱼类细胞膜的磷脂组成和力学特性。生物物理分析表明，与哺乳动物细胞系相比，鱼类细胞系具有高度可变形的细胞膜，其膜张力和杨氏模量显著降低。5-Aza-2 α -脱氧胞苷DNA去甲基化诱导细胞衰老显著降低了鱼细胞膜的可变形性，但过氧化氢诱导的氧化应激对细胞膜的可变形性没有影响。磷脂质谱分析显示，在5-Aza-2′-脱氧胞苷诱导的细胞衰老过程中，含有多不饱和脂肪酸的磷脂酰乙醇胺分子水平显著升高。鱼类细胞系为研究细胞衰老过程中细胞膜理化性质的变化提供了一个有用的模型系统。2D-TLC：二维薄层色谱；5-Aza-dC: 5-Aza-2ʹ脱氧胞苷;DHA：二十二碳六烯酸；EPA：二十碳五烯酸；胎牛血清；PC:磷脂酰胆碱;体育:磷脂酰乙醇胺;PI:磷脂酰肌醇;PS:磷脂酰丝氨酸;PUFA：多不饱和脂肪酸；SA-β-gal：衰老相关β-半乳糖苷酶；鱼类细胞具有高度可变形的细胞膜，细胞衰老的诱导显著影响细胞膜的可变形性和磷脂种类。
ABSTRACT Fish cell lines are widely used for the studies of developmental biology, virology, biology of aging, and nutrition physiology. However, little is known about their physicochemical properties. Here, we report the phospholipid compositions and mechanical properties of cell membranes derived from freshwater, anadromous and marine fish species. Biophysical analyses revealed that fish cell lines have highly deformable cell membranes with significantly low membrane tensions and Young’s moduli compared with those of mammalian cell lines. The induction of cellular senescence by DNA demethylation using 5-Aza-2ʹ-deoxycytidine significantly reduced the deformability of fish cell membrane, but hydrogen peroxide-induced oxidative stress did not affect the deformability. Mass spectrometry analysis of phospholipids revealed that the level of phosphatidylethanolamine molecules containing polyunsaturated fatty acids significantly increased during the 5-Aza-2ʹ-deoxycytidine-induced cellular senescence. Fish cell lines provide a useful model system for studying the changes in the physicochemical properties of cell membranes during cellular senescence. Abbreviations: 2D-TLC: two-dimensional thin layer chromatography; 5-Aza-dC: 5-Aza-2ʹ-deoxycytidine; DHA: docosahexaenoic acid; EPA: eicosapentaenoic acid; FBS: fetal bovine serum; PC: phosphatidylcholine; PE: phosphatidylethanolamine; PI: phosphatidylinositol; PS: phosphatidylserine; PUFA: polyunsaturated fatty acid; SA-β-gal: senescence-associated beta-galactosidase; SM: sphingomyelin Graphic abstract Fish cells have highly deformable cell membranes and induction of cellular senescence significantly affected the deformability and phospholipid species of the membrane.