CMV-specific central memory T cells reside in bone marrow
CMV-specific central memory T cells reside in bone marrow
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DOI：
10.1002/eji.200636930
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发表时间：
2007-11-01
影响因子：
5.4
通讯作者：
Scheibenbogen, Carmen
中科院分区：
文献类型：
--
作者：
Letsch, Anne;Knoedler, Maren;Scheibenbogen, Carmen
外周血（PB）中CMV特异性CD 8（+）T细胞应答的特征在于效应T细胞和效应记忆T细胞的优势。CMV特异性中枢记忆T细胞（T-CM），被认为是维持长期免疫力的关键，在PB中很少检测到。在这项研究中，我们使用细胞内细胞因子和四聚体染色分析了人PB和BM配对样本中CMV pp 65特异性CD 8（+）T细胞的分化和功能。与BM相比，PB中CMV pp 65特异性T细胞的总体频率相似;然而，CMV特异性CD 45 RA（-）CCR 7（+）T-CM几乎仅在BM中可检测到，这与T-CM在BM中的一般蓄积无关。在体外，CMV特异性T细胞可以从BM（中位数128倍，n=6）比从PB（中位数72倍，p=0.01）更有效地扩增。总之，这些数据表明BM是一个含有CMV特异性T-CM的区室，并强调了BM作为次级免疫器官的概念。CMV特异性骨髓来源的T-CM可能是产生过继转移T细胞的有价值的来源。
CMV-specific CD8(+) T cell responses in peripheral blood (PB) are characterized by a preponderance of effector and effector memory T cells. CMV-specific central memory T cells (T-CM), which are considered crucial in maintaining long-term immunity, are rarely detectable in PB. In this study we have analyzed differentiation and function of CMV pp65-specific CD8(+) T cells in paired samples of human PB and BM using intracellular cytokine and tetramer staining. Overall frequencies of CMV pp65-specific T cells were similar in PB compared to BM; however, CMV-specific CD45RA(-)CCR7(+) T-CM were almost exclusively detectable in BM, which was not related to a general accumulation of T-CM in BM. In vitro, CMV-specific T cells could be more efficiently expanded from BM (median 128-fold, n=6) than from PB (median 72-fold, p=0.01). Taken together, these data show that the BM is a compartment harboring CMV-specific T-CM and underline the concept of the BM as a secondary immune organ. CMV specific BM-derived T-CM might be a valuable source for generating T cells for adoptive transfer.