Low-carbohydrate, high-fat diet and running exercise influence bone parameters in old mice
Low-carbohydrate, high-fat diet and running exercise influence bone parameters in old mice
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低碳水化合物、高脂肪饮食和跑步运动对老年小鼠骨参数的影响
DOI：
10.1152/japplphysiol.00789.2021
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Higashida K
中科院分区：
文献类型：
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作者：
Aikawa Y;Yamashita T;Nakai N;Higashida K
我们研究了低碳水化合物、高脂肪 (LCHF) 饮食和自愿跑步运动对老年小鼠骨骼的影响和相互作用。 19 个月大的雄性小鼠根据饮食（对照组与 LCHF）和运动（久坐与自愿跑步）分为四组。按卡路里百分比计算，对照饮食为 55% 碳水化合物、23% 蛋白质和 22% 脂肪，LCHF 饮食为 10% 碳水化合物、33% 蛋白质和 57% 脂肪。当小鼠长到 24 个月大时，实验结束。使用饮食和运动的双向方差分析进行统计分析。 LCHF 饮食降低了骨矿物质含量 (BMC)、骨矿物质密度、骨体积分数和小梁数量。饮食和运动对许多骨骼参数没有显着的相互作用。然而，饮食和运动对腰椎 BMC 和胫骨小梁总组织体积和平均皮质厚度存在显着的相互作用。 LCHF 饮食削弱了跑步运动对腰椎 BMC 的益处，并导致跑步对胫骨小梁总组织体积产生负面影响。我们的研究表明，LCHF 饮食会损害老年小鼠的骨量和一些小梁微结构，并降低运动对腰椎 BMC 的益处。新的和值得注意的 LCHF 饮食用于治疗和预防疾病或改善运动表现。然而，一些研究表明，LCHF 饮食会减少幼年啮齿动物的骨质。我们的研究表明，LCHF 饮食会损害老年小鼠的骨量和一些小梁微观结构，这与之前使用年轻啮齿动物进行的研究相似。此外，我们的研究表明，LCHF 饮食会降低运动对老年小鼠腰椎 BMC 的益处。
We examined the effects and interactions of a low-carbohydrate, high-fat (LCHF) diet and voluntary running exercise on bone in older mice. Male 19-mo-old mice were divided into four groups by diet (control vs. LCHF) and exercise (sedentary vs. voluntary running). The control diet was 55% carbohydrate, 23% protein, and 22% fat, and the LCHF diet was 10% carbohydrate, 33% protein, and 57% fat as percentages of calories. The experiment ended when the mice reached 24 mo old. Statistical analysis was conducted using two-way analysis of variance with diet and exercise. The LCHF diet decreased bone mineral content (BMC), bone mineral density, bone volume fraction, and trabecular number. There was no significant interaction between diet and exercise on many bone parameters. However, there were significant diet and exercise interactions on lumbar BMC and tibial trabecular total tissue volume and average cortical thickness. The LCHF diet attenuated the benefit of running exercise on lumbar BMC and caused running to have a negative effect on tibial trabecular total tissue volume. Our study suggests that the LCHF diet impairs bone mass and some trabecular microstructure and reduces the benefit of exercise on lumbar BMC in old mice.NEW & NOTEWORTHYAn LCHF diet is used in treatment and prevention of diseases or improving exercise performance. However, some studies have shown that an LCHF diet diminishes bone in young rodents. Our study demonstrates that an LCHF diet impairs bone mass and some trabecular microstructure in old mice, which are similar to the previous studies using young rodents. Moreover, our study shows that an LCHF diet reduces the benefit of exercise on lumbar BMC in old mice.