Correlation of Blast-Induced Tympanic Membrane Perforation with Peripheral Cochlear Synaptopathy
Correlation of Blast-Induced Tympanic Membrane Perforation with Peripheral Cochlear Synaptopathy
复制标题
爆炸引起的鼓膜穿孔与周围耳蜗突触病的相关性
DOI：
10.1089/neu.2021.0487
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发表时间：
2022
影响因子：
4.2
通讯作者：
Shiotani Akihiro
中科院分区：
文献类型：
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作者：
Kurioka Takaomi;Mizutari Kunio;Satoh Yasushi;Shiotani Akihiro
听觉器官，包括鼓膜、耳蜗和中枢听觉通路，是暴露于爆炸超压时人体最脆弱的组成部分。鼓膜穿孔（TMP）是爆震暴露患者最常见的症状。然而，TMP对内耳和中枢听觉系统的影响尚未完全了解。本研究旨在分析爆震诱导的TMP对爆震后小鼠听觉病理生理变化的影响。将7周龄的小鼠暴露于爆炸超压以诱导TMP，并使其存活两个月。所有TMP病例均在爆炸暴露后3周自行愈合。与对照组相比，爆炸暴露的小鼠表现出显着的听力阈值和一个明显的干扰静纤毛的外毛细胞，无论TMP的发生或不存在。内毛细胞中突触的减少，这是已知的爆炸暴露耳蜗中最常见的病理，在没有TMP的小鼠中显著更严重。然而，无论TMP是否存在，在耳蜗核中观察到VGLUT-1标记的兴奋性中枢突触的数量减少。我们的研究结果表明，爆炸引起的TMP减轻外周耳蜗突触破坏，但离开中央听觉突触不受影响，表明中央突触破坏是独立的TMP和外周耳蜗突触破坏。外周和中枢听觉系统的突触退化可促进爆炸引起的听力障碍，包括异常听觉感知。
The auditory organs, including the tympanic membrane, cochlea, and central auditory pathway, are the most fragile components of the human body when exposed to blast overpressure. Tympanic membrane perforation (TMP) is the most frequent symptom in blast-exposed patients. The impact of TMP on the inner ear and central auditory system, however, is not fully understood. We aimed to analyze the effect of blast-induced TMP on the auditory pathophysiological changes in mice after blast exposure. Mice aged seven weeks were exposed to blast overpressure to induce TMP and allowed to survive for two months. All TMP cases had spontaneously healed by week three after the blast exposure. Compared with controls, blast-exposed mice exhibited a significant elevation in hearing thresholds and an apparent disruption of stereocilia in the outer hair cells, regardless of the occurrence or absence of TMP. The reduction in synapses in the inner hair cells, which is known as the most frequent pathology in blast-exposed cochleae, was significantly more severe in mice without TMP. A decrease in the number of excitatory central synapses labeled by VGLUT-1 in the cochlear nucleus was observed, however, regardless of the absence or presence of TMP. Our findings suggest that blast-induced TMP mitigates peripheral cochlear synaptic disruption but leaves the central auditory synapses unaffected, indicating that central synaptic disruption is independent of TMP and peripheral cochlear synaptic disruption. Synaptic deterioration in the peripheral and central auditory systems can contribute to the promotion of blast-induced hearing impairment, including abnormal auditory perception.