Synthesis of macroporous poly(dimethylsiloxane) scaffolds for tissue engineering applications.
Synthesis of macroporous poly(dimethylsiloxane) scaffolds for tissue engineering applications.
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DOI：
10.1080/09205063.2012.735097
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Stabler CL
中科院分区：
文献类型：
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作者：
Pedraza E;Brady AC;Fraker CA;Stabler CL
采用氯化钠颗粒（NaCl）和溶剂浇铸和颗粒沥滤（SCPL）技术，从聚二甲基硅氧烷（PDMS）中制备具有可控多孔结构的大孔生物稳定支架。评价了颗粒尺寸范围和总体孔隙率对所得结构的影响。结果发现，90% v/v支架和100 µm以上的颗粒范围具有最佳的开放框架和孔隙率。所得疏水性PDMS支架用纤连蛋白包被，并作为使用人间充质干细胞的贴壁细胞培养的平台进行评价。还在啮齿动物模型中评价了PDMS支架的生物相容性，其中发现植入物具有高度的生物相容性和生物稳定性，整个支架具有阳性细胞外基质沉积。这些结果证明了大孔PDMS支架对于组织工程应用的适用性，其中需要与宿主的强整合。
Macroporous, biostable scaffolds with controlled porous architecture were prepared from poly(dimethylsiloxane) (PDMS) using sodium chloride particles (NaCl) and a solvent casting and particulate leaching (SCPL) technique. The effect of particulate size range and overall porosity on the resulting structure was evaluated. Results found 90% v/v scaffolds and particulate ranges above 100 µm to have the most optimal open framework and porosity. Resulting hydrophobic PDMS scaffolds were coated with fibronectin and evaluated as a platform for adherent cell culture using human mesenchymal stem cells. Biocompatibility of PDMS scaffolds was also evaluated in a rodent model, where implants were found to be highly biocompatibile and biostable, with positive extracellular matrix deposition throughout the scaffold. These results demonstrate the suitability of macroporous PDMS scaffolds for tissue engineering applications where strong integration with the host is desired.